| Nutrient Broth |
| Nutrient Agar |
REFERENCES
1. Garrity, G.M., Boone, D.R., & Castenholz, R.W. (Eds.). (2001). Bergey’s Manual of Systematic Bacteriology: Volume 1. Springer.
2. Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C., & Yolken, R.H. (Eds.). (2003). Manual of Clinical Microbiology (8th ed.). ASM
Press.
3. Thermo Fisher Scientific (Oxoid CM0001, CM0003 Technical Data Sheets).
4. Sigma-Aldrich Microbiology Media Specifications.
DESCRIPTION
Nutrient agar and nutrient broth are general-purpose, non-selective and non-
differential microbiological culture media used for cultivation of non-fastidious
microorganisms.
Applications:
- Routine cultivation of non-fastidious bacteria (e.g., Escherichia coli, Bacillus spp.,
Pseudomonas spp.)
- Maintenance of stock cultures
- Pre-enrichment before selective media
- Viability and quality control testing.
COMPOSITION
Standard composition (per 1 liter): peptone 5.0 g, beef extract 1.0-3.0 g, sodium
chloride 5.0 g, agar 15.0 g (not for broth medium).
Add distilled water ad 1000ml; adjust pH to 7.3 ± 0.2 at 25 ºC. Sterilize by autoclaving
at 121 ºC for 15 minutes.
Some formulations may include yeast extract (~2 g/L) depending on manufacturer.
For agar: cool to 45-50 ºC and pour into sterile Petri dishes.
QUALITY CONTROL
Escherichia coli ATCC 25922
Staphylococcus aureus ATCC 25923
Pseudomonas aeruginosa ATCC 27853.
Nutrient agar and nutrient broth are general-purpose, non-selective and non-
differential microbiological culture media used for cultivation of non-fastidious
microorganisms.
Applications:
- Routine cultivation of non-fastidious bacteria (e.g., Escherichia coli, Bacillus spp.,
Pseudomonas spp.)
- Maintenance of stock cultures
- Pre-enrichment before selective media
- Viability and quality control testing.
COMPOSITION
Standard composition (per 1 liter): peptone 5.0 g, beef extract 1.0-3.0 g, sodium
chloride 5.0 g, agar 15.0 g (not for broth medium).
Add distilled water ad 1000ml; adjust pH to 7.3 ± 0.2 at 25 ºC. Sterilize by autoclaving
at 121 ºC for 15 minutes.
Some formulations may include yeast extract (~2 g/L) depending on manufacturer.
For agar: cool to 45-50 ºC and pour into sterile Petri dishes.
QUALITY CONTROL
Escherichia coli ATCC 25922
Staphylococcus aureus ATCC 25923
Pseudomonas aeruginosa ATCC 27853.
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