(c) Costin Stoica
Measured diameter of the inhibition zones are compared
with the standard values and sorted in descending order
of antibacterial activity (also bacterial strain is marked as
'susceptible', 'intermediate' or 'resistant' accordingly to the
Software database contains CLSI and EUCAST
recommended antibiotics for Enterobacteriaceae,
Pasteurellaceae, Enterococcus, Neisseria,
Staphylococcus, Streptococcus and Pseudomonas.
The project started in 2003 as a MS-DOS-based program
for clinically significant Enterobacteriaceae identification,
using biochemical characters, morphology, growth
conditions, ecology and pathogenicity data.
In one year program reached version 4, upgrading with 3
more genera: Bacillus, Streptococcus & Staphylococcus.
ABIS 5 was released in 2005 with major changes
including an easy to use Windows based interface, step
by step identification for Gram positive & negative bacteria,
rods / cocci (Genera only), identification maps and 2 more
families included: Pasteurellaceae (Pasteurella,
Actinobacillus, Haemophilus) and Pseudomonadaceae.
Also, Bacillus, Enterobacteriaceae & Staphylococcus
databases were updated and the biochemical tests list,
used for identification, was extended.
Continuous changes in bacterial taxonomy forced us to
stop the development of the stand-alone version and
focus on the online development. In the year 2007 "ABIS 6
online", linked with a germs Encyclopedia and hosted by
'Regnum Prokaryotae" site, was officially released for
public use. Later, ABIS 7 brought Vibironaceae
identification, ABIS 8 - family Campylobacteraceae and
ABIS 9 - genus Clostridium and Salmonella
Kauffman-White scheme. Other upgrades followed up to
actual ABIS: Helicobacter and Lactobacillus (ABIS 10),
Listeria and Erysipelothrix (ABIS 11), Corynebacterium
and Lancefield grouping for streptococci (ABIS 12),
Neisseria and Chromobacterium (ABIS13).
If you want to know more, then check out the development
log [here] or watch the video tutorial [here].
ABIS online - Advanced Bacterial Identification Software is a laboratory tool for bacterial identification, based
on morpho-biochemical characters, cultural characteristics, growth conditions, ecology and pathogenicity
data. The program allows a great flexibility in choosing biochemical tests and is an alternative to
commercial systems or code-books. The software is linked with a bacterial Encyclopedia, the Kauffman-
White scheme for Salmonella serovars identification, streptococci Lancefield grouping, and an antibiogram
interpreter (diffusimetric method).
Main identifiable taxa: Enterobacteriaceae, Pasteurellaceae, Campylobacteraceae, Bacillaceae,
Lactobacillus, Staphylococcus, Streptococcus, Clostridium, Vibrio, Listeria, Erysipelothrix, Mycobacterium,
Corynebacterium, Neisseria, Chromobacterium, Aeromonas, Pseudomonas and related species. The
number of taxa included in database is constantly expanding, and at the time I wrote this article, the
database had exceeded 1,900 species.
Android Independent Apps
Regnum Prokaryotae Encyclopedia, ABIS online and Antibiogram interpreter can be downloaded and
installed as free applications, outside the Google play store. If your mobile device does not allow installing
from 3rd party sources, then follow the steps below:
1. Go to "Settings",
2. Go to "Security" or "Lock screen and security",
3. Scroll down and check “Unknown sources” box,
4. Tap ‘OK” when the warning pops up,
5. Download APK and install it.
For Android 8.0 or later go to “Settings => Apps & notifications => Advanced => Special app access =>
Install unknown apps.” Here, select the app (usually your Internet browser) that you want to grant
permission to install from unknown sources. Then once you’ve tapped it, tap “Allow from this source” to
enable unknown sources for that specific app.
ABIS online - Bacterial identification app
ATI - Antibiogram Test Interpreter app
Regnum Prokaryotae Encyclopedia app