Inoculate the plates and incubate aerobically,18 – 24 hours, at 35°C.
Sucrose-fermenting vibrios appear smooth, opaque, thin-edged yellow colonies. Non-sucrose-fermenting colonies appear green.
Growth of Enterococcus faecalis ATCC 29212 & Escherichia coli ATCC 25922 is inhibited.
Vibrio alginolyticus ATCC 17749 & Vibrio cholerae ATCC 9459 produce yellow colonies.
Vibrio parahaemolyticus ATCC 17802 produces green colonies.
Pseudomonas aeruginosa ATCC 27853 is partially to completely inhibited; forms blue colonies.
Yeast extract 0,5 % (vol), Peptone 1,0%, Na thiosulphate1,0%, Sodium citrate 1,0%,
Bile 0,8%, Saccharose 2,0%, NaCl 1,0%, Fe citrate 0,1%, Bromothymol blue 0,004%,
Thymol blue 0,004%, Agar 1,4%.
Adjust pH to 8.6, bring to boiling temperature. Do not autoclave.
Sodium Citrate, Sodium Thiosulfate, Sodium Cholate, and Ox bile inhibit
Gram-positive organisms and suppress coliforms. An increased pH is used to
favorize the growth of vibrios. Sucrose is the fermentable carbohydrate. Sodium
Chloride stimulates organism growth and maintains osmotic balance of the medium.
Sodium Thiosulfate is also a sulfur source, and acts with Ferric Citrate as an indicator
to detect hydrogen sulfide production. Bromthymol Blue and Thymol Blue are pH
Medium frequently used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios.
(c) Costin Stoica