| Mycobacterium bovis in Sauton medium |
REFERENCES
1. Giampaglia CM, de Brito AC, Martins MC, Ueki SY, Latrilha FO, de Oliveira RS,
Yamauchi JU, da Silva Telles MA. 2009. Maintenance of Mycobacterium tuberculosis
on glass beads. Ann Clin Lab Sci 39:51–54.
2. Guallar-Garrido, Sandra & Campo-Pérez, Víctor & Sánchez-Chardi, Alejandro &
Luquin, Marina & Julián, Esther. (2020). Each Mycobacterium Requires a Specific
Culture Medium Composition for Triggering an Optimized Immunomodulatory and
Antitumoral Effect. Microorganisms. 8. 734. 10.3390/microorganisms8050734.
3. Himedia Laboratories. Sautons Fluid Medium Base - technical data.
1. Giampaglia CM, de Brito AC, Martins MC, Ueki SY, Latrilha FO, de Oliveira RS,
Yamauchi JU, da Silva Telles MA. 2009. Maintenance of Mycobacterium tuberculosis
on glass beads. Ann Clin Lab Sci 39:51–54.
2. Guallar-Garrido, Sandra & Campo-Pérez, Víctor & Sánchez-Chardi, Alejandro &
Luquin, Marina & Julián, Esther. (2020). Each Mycobacterium Requires a Specific
Culture Medium Composition for Triggering an Optimized Immunomodulatory and
Antitumoral Effect. Microorganisms. 8. 734. 10.3390/microorganisms8050734.
3. Himedia Laboratories. Sautons Fluid Medium Base - technical data.
COMPOSITION
Sauton's medium
KH2PO4 0.5 g; MgSO4 * 7H2O, 0.5 g; citric acid, 2.0 g; ferric ammonium citrate, 0.05
g; glycerol, 60 ml; sodium glutamate, 4.0 g; distilled water ad 1 L.
Dissolve the reagents, except glycerol, in 300 ml of water using heat. Add glycerol
then bring the volume to 1 L with distilled water. Adjust pH to 7.4 with 1 M NaOH.
Autoclave at 121 ºC for 15 min.
NOTE
Adding ZnSO4 * 7H2O, 1% solution 1ml/L of medium improves growth of some
Mycobacteria.
Variants
Sautons Fluid Medium Base by HiMedia
ferric ammonium citrate (brown) 0.0167 g, L-asparagine 1.33 g, citric acid 0.66 g,
MgSO4 * 7H2O 0.166 g, dipotassium hydrogen phosphate 0.177 g, sodium
dihydrogen phosphate 0.056 g, sodium chloride 0.035 g, polysorbate 80 0.833 g,
distilled water ad 1000 ml.
Adjust pH (at 25 ºC) to 7.2±0.2. Sterilize by autoclaving at 121 ºC for 15 minutes.
PROCEDURE
Inoculate a loopful of Mycobacterium culture and place it on medium surface. Do not
let the culture sunk (admin note).
QUALITY CONTROL
Mycobacterium smegmatis ATCC 14468: good growth;
Mycobacterium tuberculosis H37RV(25618): good growth.
Sauton's medium
KH2PO4 0.5 g; MgSO4 * 7H2O, 0.5 g; citric acid, 2.0 g; ferric ammonium citrate, 0.05
g; glycerol, 60 ml; sodium glutamate, 4.0 g; distilled water ad 1 L.
Dissolve the reagents, except glycerol, in 300 ml of water using heat. Add glycerol
then bring the volume to 1 L with distilled water. Adjust pH to 7.4 with 1 M NaOH.
Autoclave at 121 ºC for 15 min.
NOTE
Adding ZnSO4 * 7H2O, 1% solution 1ml/L of medium improves growth of some
Mycobacteria.
Variants
Sautons Fluid Medium Base by HiMedia
ferric ammonium citrate (brown) 0.0167 g, L-asparagine 1.33 g, citric acid 0.66 g,
MgSO4 * 7H2O 0.166 g, dipotassium hydrogen phosphate 0.177 g, sodium
dihydrogen phosphate 0.056 g, sodium chloride 0.035 g, polysorbate 80 0.833 g,
distilled water ad 1000 ml.
Adjust pH (at 25 ºC) to 7.2±0.2. Sterilize by autoclaving at 121 ºC for 15 minutes.
PROCEDURE
Inoculate a loopful of Mycobacterium culture and place it on medium surface. Do not
let the culture sunk (admin note).
QUALITY CONTROL
Mycobacterium smegmatis ATCC 14468: good growth;
Mycobacterium tuberculosis H37RV(25618): good growth.
DESCRIPTION
liquid medium used for the cultivation and enumeration of Mycobacteria.
liquid medium used for the cultivation and enumeration of Mycobacteria.
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