PROCEDURE
Disperse culture or pathologic material on the medium surface, then incubate 2-3 days at 37 ºC .
RESULTS
Bordetella colonies are smooth, raised, shiny, 1-2 mm in diameter, sometimes with a gray-black center or surrounded by hemolysis
zone.
REFERENCES
1. Handbook Culture Media Merck, 1982.
2. Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de microbiologie clinica
ed. III, 2009, 1045-1100.
Disperse culture or pathologic material on the medium surface, then incubate 2-3 days at 37 ºC .
RESULTS
Bordetella colonies are smooth, raised, shiny, 1-2 mm in diameter, sometimes with a gray-black center or surrounded by hemolysis
zone.
REFERENCES
1. Handbook Culture Media Merck, 1982.
2. Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de microbiologie clinica
ed. III, 2009, 1045-1100.
COMPOSITION
Potatoes infusion 4.5 g, NaCl 5.5 g, Agar-agar 15 g, Glycerol 10 ml, Blood (sheep,
rabbit or human) 150-200 ml, Penicillin 250 IU.
Distilled water ad 1000 ml, pH 6.6 ± 0.2, autoclave before adding blood, pour in
plates. Plates can be stored refrigerated for 2 weeks.
Addition of 1% peptone (5 g/l peptone from casein & 5 g/l peptone from meat) without
penicillin improves the growth of Bordetella in large vaccine cultures.
Potatoes infusion preparation: wash and peel the potatoes then slice them in small
pieces. Add the NaCl and potatoes in 500 ml water. Boil 30 minutes. Filter then
adjust pH to 7.0 .
Potatoes infusion 4.5 g, NaCl 5.5 g, Agar-agar 15 g, Glycerol 10 ml, Blood (sheep,
rabbit or human) 150-200 ml, Penicillin 250 IU.
Distilled water ad 1000 ml, pH 6.6 ± 0.2, autoclave before adding blood, pour in
plates. Plates can be stored refrigerated for 2 weeks.
Addition of 1% peptone (5 g/l peptone from casein & 5 g/l peptone from meat) without
penicillin improves the growth of Bordetella in large vaccine cultures.
Potatoes infusion preparation: wash and peel the potatoes then slice them in small
pieces. Add the NaCl and potatoes in 500 ml water. Boil 30 minutes. Filter then
adjust pH to 7.0 .
DESCRIPTION
Medium was designed for the isolation of Bordetella pertusis, Bordetella parapertussis and other Bordetella species.
Penicillin addition inhibits the growth of associate Gram-positive microflora.
Medium was designed for the isolation of Bordetella pertusis, Bordetella parapertussis and other Bordetella species.
Penicillin addition inhibits the growth of associate Gram-positive microflora.
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