Ashby′s Mannitol Agar
Azotobacter chroococcum white colonies on Ashby
medium after 4 days incubation at 28 ºC (down) and
brown-pigmentation after 10 days incubation (up)
REFERENCES
  1. Himedia Laboratories. Ashby’s Mannitol Agar (M706) -  technical data.
    Available at https://himedialabs.com/TD/M706.pdf.
  2. Merck Millipore. Ashby’s Mannitol Agar (A1840) -  technical data. Available at
    https://www.sigmaaldrich.com/
  3. Costin Stoica Azotobacter chroococcum in  ABIS Encyclopedia, Regnum
    Prokaryotae. Available at https://www.tgw1916.
    net/Pseudomonas/azotobacter_chroococcum.html
STANDARD COMPOSITION
Mannitol 20.0 g, potasium phosphate dibasic 0.2 g, magnesium sulfate 0.2 g, sodium
chloride 0.2 g, potassium sulfate, 0.1 g, calcium carbonate, 5.0 g, agar 15.0 g.
Distilled water ad 1000 ml, adjust pH to 7.4 ± 0.2.

MODIFIED MEDIUM (unpublished data - admin note)
D-glucose 5.0 g, mannitol, 5.0 g, K
2HPO4 0.9 g, KH2PO4 0.1 g, MgSO4 * 7H2O 0.1 g,
NaCl 0.2 g, FeSO
4 *7H2O 0.01 g, Na2MoO4 * 2H2O 0.005 g, CaCO3 5.0 g, CaCl2 *2H2O
0.1 g, agar 15.0 g.
Distilled water ad 1000 ml, adjust pH to 7.3.


PROCEDURE
Sterilize by autoclaving at 121 ºC for 15 minutes. Cool to 45-50 ºC. Mix well and pour
into sterile Petri plates.


NOTE
Due to presence of calcium carbonate, the prepared medium forms opalescent
solution with white precipitate.
DESCRIPTION
Medium for the
isolation and cultivation of Azotobacter spp. that use mannitol as a source of carbon. Azotobacter is nitrogen fixing
bacteria which can use mannitol as a carbon source and atmospheric nitrogen as nitrogen source.
Ashby′s Mannitol Agar
(c) Costin Stoica
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