Following microorganisms should have a good to very good growth: Salmonella typhimurium ATCC 14028, Escherichia coli ATCC
25922, Enterococcus faecalis ATCC 33186, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus
ATCC 6538Candida albicans ATCC 2091, Aspergillus brasiliensis ATCC 16404.
Proteose peptone 10g, NaCl 5 g, Disodic phosphate x 12H2O 3.5 g, Monopotasic
phosphate 1.5 g, H2O ad 1000 ml; adjust pH to 7.2.
The prepared broth is clear and yellowish. Adjust pH to 7.0 ± 0.2 at 25 ºC. Sterilize by
autoclaving at 121 ºC for 15 minutes.
Medium used in the pre-enrichement step of Salmonella species isolation from foods and water, accordingly to SR EN ISO 6579
standard "Horizontal method for the detection of Salmonella spp.". It supports resuscitation and multiplication of the germs that have
been affected by unfavorable conditions like pH, temperature, drying, high osmotic pressure or preservatives.
(c) Costin Stoica