Campylobacter selective agar
Peptone protein mixture .................................................... 21 g/l
Electrolyte ........................................................................... 5 g/l
Starch, soluble .................................................................... 1 g/l
Agar-Agar .......................................................................... 13 g/l
Corynebacterium Agar
Casein peptone, tryptic digest ......................................... 10.0 g
Yeast extract ..................................................................... 5.0 g
Glucose ............................................................................. 5.0 g
NaCl .................................................................................. 5.0 g
Agar ................................................................................ 15.0 g
Distilled water ad 1000.0 ml, adjust pH to 7.2 - 7.4.
Haemophilus medium
Distilled water ...............................................................  900.0 ml
PPLO agar (Difco B412) .................................................. 34.0 g  
or Mycoplasma Agar Base (Oxoid CM 401) ................... 35.0 g
Glucose ............................................................................. 1.0 g
Sterilize at 121 °C for 15 minutes, cool to 45-50 °C and add:
Horse serum (inactivated) ................................................ 50.0 ml
Yeast extract fresh ......................................................... 100.0 ml
Suspend ingredients in distilled water and bring to the boil to dissolve completely. Adjust to pH 7.4 and add agar;
sterilize, cool to 45 - 50 °C and add:
L-Cysteine HCl ................................................................... 0.5 g/l
Vitamin K1 ........................................................................ 1.0 ml/l
Heterotrophic medium for Hydrogenomonas
KH2PO4 ..............................................................................0.2 g
MgSO4 ...............................................................................0.1 g
Sodium citrate . 2H2O ........................................................0.5 g
Sodium acetate . 3H2O ......................................................0.3 g
Sodium succinate . 6H2O ...................................................2.0 g
Sodium glutamate...............................................................1.0 g
Agar...................................................................................15.0 g
Tryptose...............................................................................5.0 g
Yeast extract........................................................................1.0 g
Corn starch...........................................................................2.0 g
Distilled water.......................................................................1.0 l
Adjust pH to 6.8-7.2. Autoclave 15 minutes at 121C.
ABM (Antarctic Bacterial Medium) Reddy et al., 2002, 2003
Peptone................................................................................ 5 g
Yeast extract ........................................................................ 2 g
Agar ....................................................................................15 g
Distilled water ad 1000 ml. Adjust pH to 6.9. Used for psychrophilic pseudomonads isolation.
Nutrient Seawater Agar
3ml of glycerol, 1 g of yeast extract, 3 g of peptone, and 15 g of agar per liter of 75% seawater.
Medium is used for marine Vibrio isolation.
Czapek - Dox Agar
Sucrose ...............................................................................30.00 g
NaNO3 ..................................................................................3.00 g
MgSO4 x 7 H2O.....................................................................0.50 g
KCl........................................................................................ 0.50 g
FeSO4 x 7 H2O .....................................................................0.01 g
K2HPO4 ................................................................................1.00 g
Agar ....................................................................................13.00 g
Distilled water 1000.00 ml; adjust pH to 7.2.
PPLO broth base
Beef heart infusion ..........................................................  6.0 g/L
Sodium chloride .............................................................. 5.0 g/L
Peptic digest of animal tissue .........................................10.0 g/L
Crystal violet ................................................................... 0.01 g/L
Final pH 7.8 For Mycoplasma isolation add 2.8 ml/l of 1% Potassium Tellurite solution and 25% Ascitic fluid .
For PPLO agar  use the same ingredients and add 14.0 g/L agar
TY Broth

Casein enzymatic hydrolysate type I ......................8g
Yeast extract powder .............................................5g
NaCl ........................................................,,,,,,,.......5g
H2O ad 1,000 ml
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BCSA medium
NaCl : 5g Saccharose : 10g Lactose : 10g Phennol red: 0,08g Crystal violet : 0,002g Trypticase peptone : 10g Yeast
extract : 1,5g  Agar : 14g  Sulfate de polymyxine B : 600 000 UI Gentamicine : 0,01 Vancomycine : 0,025 H2O ad
1000 ml. Used for Burkholderia cepacia isolation.
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Bordet-Gengou Medium
Bordet-Gengou Agar Base (BD 248200)…………………….30.0 g
Glycerol………………………………………………….………10.0 ml
Proteose peptone …….....……………………………….……..10.0 g
H2O  ......…………………………………….……………………840 ml
Adjust pH to 6.7 +/- 0.2. Autoclave at 121º C. Cool  to 45-50ºC and add 150 ml sterile defibrinated rabbit blood. Used
for Bordetella pertussis & Pseudomonas pertucinogena cultivation.
CMC agar medium (g/liter)
Carboxymethylcellulose ..........................................................1g
Peptone ..................................................................................5g
Yeast extract ...........................................................................1g
K2HPO4 .................................................................................4g
MgSO4 . 7H2O .......................................................................1g
KCl ......................................................................................0.2g
FeSO4 . 7H2O ...................................................................0.02g
Agar .....................................................................................15g
pH adjusted to 7.0. Used for Paenibacillus cellulositrophicus isolation.
Deoxyribonuclease Agar
NaCl ........................................................................................ 5.0 g
Casein enzymic hydrolysate .................................................. 15.0 g
Papaic digest of soyabean meal .............................................. 5.0 g
Deoxyribonucleic acid (DNA) ................................................... 2.0 g
Agar ....................................................................................... 15.0 g
Toluidine blue 0.25% solution ................................................. 40 ml
Distilled water ........................................................................ 960 ml
Dissolve ingredients in water by heating & mixing frequently; boil 1 minute. Adjust to pH 7.3. Autoclave 15 min,
121°C. Medium is used for the detection of deoxyribonuclease activity of bacteria and fungi, and especially for
identification of pathogenic staphylococci. It is useful for differentiation between Serratia and Enterobacter,
Moraxella and Branhamella and other Gram-negative nonfermentative microorganisms.
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