| Streptococcus canis chained cells in BHI broth, Gram staining. |
| Streptococcus septicaemia in Terra Nova dogs - short communication - DVM Costin Stoica |
The necropsy show pleuritis, blood and serum collection in
thorax and pericardum, and spotted skin.
Bacteriological exam has isolated Streptococcus canis from
heart, lungs, liver and bone marrow.
Morphological and biochemical properties:
After 24h incubation at 37 ºC on sheep blood agar, colonies
were 2-3 mm in diameter, white-gray and beta-hemolytic.
No growth on MacConkey agar, Chapman agar and
Bile-esculin agar.
Aerobic, facultatively anaerobic.
API 20 Strep, API 20 A and API 50 CHB strips (bioMerieux)
were used for the biochemical characterisation. Positive
results were obtained for arginine dihydrolase, alkaline
phosphatase and hippurate hydrolysis.
Acid was produced from fructose, glucose, ribose,
galactose, mannose, cellobiose, maltose, lactose,
N-acetylglucosamine, salicin, saccharose, trehalose and
starch.
Negative results for catalase, alpha- and
beta-galactosidase, beta-glucuronidase, pyrolidonil
arylamydase, oxidase, urease and Voges-Proskauer test.
Acid was not produced from glycerol, erythritol, D- and
L-arabinose, D- and L- xylose, adonitol, rhamnose, dulcitol,
inositol, mannitol, sorbitol, amygdalin, arbutin, esculin,
inulin, melezitose, raffinose, glycogen, xylitol, gentibiose,
turanose, lyxose, tagatose, D- and L-fucose, gluconate, 2-
and 5-ketogluconate.
Esculin hydrolysis was negative in API 20 Strep and positive
after 48h incubation in API 20 A.
Using "ABIS online" software the strain was identified as
Streptococcus canis 91%.
thorax and pericardum, and spotted skin.
Bacteriological exam has isolated Streptococcus canis from
heart, lungs, liver and bone marrow.
Morphological and biochemical properties:
After 24h incubation at 37 ºC on sheep blood agar, colonies
were 2-3 mm in diameter, white-gray and beta-hemolytic.
No growth on MacConkey agar, Chapman agar and
Bile-esculin agar.
Aerobic, facultatively anaerobic.
API 20 Strep, API 20 A and API 50 CHB strips (bioMerieux)
were used for the biochemical characterisation. Positive
results were obtained for arginine dihydrolase, alkaline
phosphatase and hippurate hydrolysis.
Acid was produced from fructose, glucose, ribose,
galactose, mannose, cellobiose, maltose, lactose,
N-acetylglucosamine, salicin, saccharose, trehalose and
starch.
Negative results for catalase, alpha- and
beta-galactosidase, beta-glucuronidase, pyrolidonil
arylamydase, oxidase, urease and Voges-Proskauer test.
Acid was not produced from glycerol, erythritol, D- and
L-arabinose, D- and L- xylose, adonitol, rhamnose, dulcitol,
inositol, mannitol, sorbitol, amygdalin, arbutin, esculin,
inulin, melezitose, raffinose, glycogen, xylitol, gentibiose,
turanose, lyxose, tagatose, D- and L-fucose, gluconate, 2-
and 5-ketogluconate.
Esculin hydrolysis was negative in API 20 Strep and positive
after 48h incubation in API 20 A.
Using "ABIS online" software the strain was identified as
Streptococcus canis 91%.
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(c) Costin Stoica
| R E G N U M PROKARYOTAE |
| Serous pleuritis, clear red-pink fluid collection |
| Beta-hemolytic colonies on Sheep Blood Agar. |
Streptococcus canis is a pathogenic bacteria that can produce in dogs and cats dermatitis, pharyngitis,
sinusitis, septicaemia, otitis, vaginitis and metritis. In bovines may produce mammitis. It is rarely isolated
from humans, but may be involved in septicaemic infections.
In this case all, puppies from three females belonging to the same breeder, died after 1 to 4 days from birth.
The laboratory received a recently died, 4-days-old, puppy.
sinusitis, septicaemia, otitis, vaginitis and metritis. In bovines may produce mammitis. It is rarely isolated
from humans, but may be involved in septicaemic infections.
In this case all, puppies from three females belonging to the same breeder, died after 1 to 4 days from birth.
The laboratory received a recently died, 4-days-old, puppy.
Lancefield grouping was determined by latex agglutination
(Streptococcal Grouping Kit, Oxoid). The strain reacted with
Lancefield Group G antisera.
Antibiogram results: sensible to Gentamicin, Florfenicol,
Fosfomycin, Doxycycline, Amoxicillin, Ampicillin, Lincomycin,
Furazolidone, Enrofloxacine, Erithromycin; resistant to
Neomycin, Sulphamethoxazole+Trimethoprim and
Oxitetracycline.
(Streptococcal Grouping Kit, Oxoid). The strain reacted with
Lancefield Group G antisera.
Antibiogram results: sensible to Gentamicin, Florfenicol,
Fosfomycin, Doxycycline, Amoxicillin, Ampicillin, Lincomycin,
Furazolidone, Enrofloxacine, Erithromycin; resistant to
Neomycin, Sulphamethoxazole+Trimethoprim and
Oxitetracycline.
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