| Pseudomonas aeruginosa isolated from Chinchilla - short communication - Dr. Costin Stoica |
The lab received 2 dead gray chinchillas (Chinchilla lanigera),
one young and one adult female, from a small breeding farm.
Reported mortality in the farm was 5 dead out of 80 individuals.
Death was rapid and no clinical signs were observed.
Necropsy revealed in both cases intense white spotted liver
(picture 1). Same types of spotted areas was observed on
external side of the intestine (picture 2).
Bacteriology:
One blood agar and one Mac Conkey agar plate were
inoculated with liver samples and incubated 24 hours at 37 ºC.
On both plates from the adult female was obtained a pure
culture: on blood agar - wrinkled, elongated, haemolytic
colonies and on Mac Conkey agar lactose negative colonies.
Morphological and biochemical properties of the strain:
Pigment production was investigated on Mueller-Hinton agar.
After 48 hours of incubation a green, fluorescent, diffusible
pigment appeared.
Anaerobic growth failed. Growth at 41 ºC, oxidase and catalase
positive.
Gram-negative rods.
TSI, MIU and Simmons results:
TSI - strictly aerobic, lactose-sucrose negative
MIU - growth failed
Simmons - citrate positive
Biochemical results on API 20 NE strip:
Nitrates and nitrites were reduced. Indole production, glucose
acidification, arginine dihydrolase, urease, esculin & PNPG
were negative. Gelatin hydrolysis was positive. Assimilation of
glucose, mannitol, N-acetyl-glucosamine, gluconate, caprate,
adipate, malate & citrate were positive. Assimilation of
arabinose, mannose, maltose and phenyl-acetate were
negative.
API identified strain as Pseudomonas aeruginosa 99.9%.
With ABIS 6 online software result was Pseudomonas
aeruginosa 85% (with a question mark on the negative arginine
dihydrolase).
one young and one adult female, from a small breeding farm.
Reported mortality in the farm was 5 dead out of 80 individuals.
Death was rapid and no clinical signs were observed.
Necropsy revealed in both cases intense white spotted liver
(picture 1). Same types of spotted areas was observed on
external side of the intestine (picture 2).
Bacteriology:
One blood agar and one Mac Conkey agar plate were
inoculated with liver samples and incubated 24 hours at 37 ºC.
On both plates from the adult female was obtained a pure
culture: on blood agar - wrinkled, elongated, haemolytic
colonies and on Mac Conkey agar lactose negative colonies.
Morphological and biochemical properties of the strain:
Pigment production was investigated on Mueller-Hinton agar.
After 48 hours of incubation a green, fluorescent, diffusible
pigment appeared.
Anaerobic growth failed. Growth at 41 ºC, oxidase and catalase
positive.
Gram-negative rods.
TSI, MIU and Simmons results:
TSI - strictly aerobic, lactose-sucrose negative
MIU - growth failed
Simmons - citrate positive
Biochemical results on API 20 NE strip:
Nitrates and nitrites were reduced. Indole production, glucose
acidification, arginine dihydrolase, urease, esculin & PNPG
were negative. Gelatin hydrolysis was positive. Assimilation of
glucose, mannitol, N-acetyl-glucosamine, gluconate, caprate,
adipate, malate & citrate were positive. Assimilation of
arabinose, mannose, maltose and phenyl-acetate were
negative.
API identified strain as Pseudomonas aeruginosa 99.9%.
With ABIS 6 online software result was Pseudomonas
aeruginosa 85% (with a question mark on the negative arginine
dihydrolase).
| Image 1 - liver lesions |
Antibiogram:
The isolated strain was sensible to Enrofloxacin, Gentamicin, Neomycin, Streptomycin, Kanamycin and resistant to Lincomycin,
Amoxicillin, Bacitracin, Sulphamethoxazole+Trimethoprim, Ampicillin, Trimethoprim, Penicillin, Tetracycline.
Taking into consideration bacteriological and necropsy results we may conclude that the Pseudomonas aeruginosa septicaemia was
the main cause of death.
The pseudomonosis episode was stopped with Enrofloxacin treatment.
The isolated strain was sensible to Enrofloxacin, Gentamicin, Neomycin, Streptomycin, Kanamycin and resistant to Lincomycin,
Amoxicillin, Bacitracin, Sulphamethoxazole+Trimethoprim, Ampicillin, Trimethoprim, Penicillin, Tetracycline.
Taking into consideration bacteriological and necropsy results we may conclude that the Pseudomonas aeruginosa septicaemia was
the main cause of death.
The pseudomonosis episode was stopped with Enrofloxacin treatment.
| Image 2 - intestinal lesions |
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