| Deadly Klebsiella pneumoniae in canaries - short communication - DVM Costin Stoica |
One canaries breeder reported a high mortality in population; an approximate of 25% deaths
of various sexes and ages, mostly young birds. First observed clinical sign was leg arthritis
followed in 1-2 weeks by necrosis and death. Phenomena was happening 2 years in a row
with periods of remission. 3 canary corpses were brought for lab investigations.
Necropsy showed white-yellowish spotted liver and leg necrosis with a desquamated aspect of
the skin. Parasitology exam was negative. Bacteriology found 2 strains of Klebsiella
penumoniae subsp. pneumoniae from liver and leg with necrosis. Both strains were
biochemically identical.
Morphological and biochemical properties of the strains:
On blood agar: S-type colonies, nonhemolytic, 2-3 mm in diameter.
On MacConkey agar: lactose positive colonies
On Mueller-Hinton agar: nonpigmented, smooth colonies.
Aerobic, facultatively anaerobic, Gram-negative, nonmotile rods.
Catalase positive & oxidase negative.
of various sexes and ages, mostly young birds. First observed clinical sign was leg arthritis
followed in 1-2 weeks by necrosis and death. Phenomena was happening 2 years in a row
with periods of remission. 3 canary corpses were brought for lab investigations.
Necropsy showed white-yellowish spotted liver and leg necrosis with a desquamated aspect of
the skin. Parasitology exam was negative. Bacteriology found 2 strains of Klebsiella
penumoniae subsp. pneumoniae from liver and leg with necrosis. Both strains were
biochemically identical.
Morphological and biochemical properties of the strains:
On blood agar: S-type colonies, nonhemolytic, 2-3 mm in diameter.
On MacConkey agar: lactose positive colonies
On Mueller-Hinton agar: nonpigmented, smooth colonies.
Aerobic, facultatively anaerobic, Gram-negative, nonmotile rods.
Catalase positive & oxidase negative.
TSI, MIU and Simmons results:
TSI - Glucose, Lactose & Sacharose fermented.
Gas produced from Glucose. H2S not produced.
MIU - Indole, Urea & motility negative
Simmons - citrate positive
Biochemical results on API 20 E strip:
ONPG, Citrate, Urea, Voges-Proskauer, Glucose,
Mannose, Inositol, Sorbitol, Rhamnose,
Sacharose, Melibiose, Amylase, Arabinose
positive. Urea became positive after 48 hours.
Nitrates reduced.
Arginine dihydrolase, Lysine decarboxylase,
Ornithine decarboxylase, H2S, TDA, Indole,
Gelatin liquefaction negative.
TSI - Glucose, Lactose & Sacharose fermented.
Gas produced from Glucose. H2S not produced.
MIU - Indole, Urea & motility negative
Simmons - citrate positive
Biochemical results on API 20 E strip:
ONPG, Citrate, Urea, Voges-Proskauer, Glucose,
Mannose, Inositol, Sorbitol, Rhamnose,
Sacharose, Melibiose, Amylase, Arabinose
positive. Urea became positive after 48 hours.
Nitrates reduced.
Arginine dihydrolase, Lysine decarboxylase,
Ornithine decarboxylase, H2S, TDA, Indole,
Gelatin liquefaction negative.
API identified strain as Klebsiella penumoniae subsp. pneumoniae 98%
With ABIS 6 online software result was Klebsiella penumoniae subsp. pneumoniae 91% (with a
question mark on the negative Lysine decarboxylase). Next choice in both identification
systems was Klebsiella planticola.
Both isolated strains were sensible to Colistin sulphate, intermediate to Gentamicin, Neomycin
and resistant to Enrofloxacin, Streptomycin, Kanamycin, Lincomycin, Amoxicillin, Bacitracin,
Sulphamethoxazole+Trimethoprim, Ampicillin, Trimethoprim, Spectinomycin & Tetracycline.
With ABIS 6 online software result was Klebsiella penumoniae subsp. pneumoniae 91% (with a
question mark on the negative Lysine decarboxylase). Next choice in both identification
systems was Klebsiella planticola.
Both isolated strains were sensible to Colistin sulphate, intermediate to Gentamicin, Neomycin
and resistant to Enrofloxacin, Streptomycin, Kanamycin, Lincomycin, Amoxicillin, Bacitracin,
Sulphamethoxazole+Trimethoprim, Ampicillin, Trimethoprim, Spectinomycin & Tetracycline.
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