| Deadly Klebsiella pneumoniae in canaries - short communication - DVM Costin Stoica |
One canaries breeder reported a high mortality in population; an approximate of 25% deaths of various sexes
and ages, mostly young birds. First observed clinical sign was leg arthritis followed in 1-2 weeks by necrosis
and death. Phenomena was happening 2 years in a row with periods of remission. 3 canary corpses were
brought for lab investigations.
Necropsy showed white-yellowish spotted liver and leg necrosis with a desquamated aspect of the skin.
Parasitology exam was negative. Bacteriology found 2 strains of Klebsiella penumoniae subsp. pneumoniae
from liver and leg with necrosis. Both strains were biochemically identical.
Morphological and biochemical properties of the strains:
On blood agar: S-type colonies, nonhemolytic, 2-3 mm in diameter.
On MacConkey agar: lactose positive colonies
On Mueller-Hinton agar: nonpigmented, smooth colonies.
Aerobic, facultatively anaerobic, Gram-negative, nonmotile rods.
Catalase positive and oxidase negative.
and ages, mostly young birds. First observed clinical sign was leg arthritis followed in 1-2 weeks by necrosis
and death. Phenomena was happening 2 years in a row with periods of remission. 3 canary corpses were
brought for lab investigations.
Necropsy showed white-yellowish spotted liver and leg necrosis with a desquamated aspect of the skin.
Parasitology exam was negative. Bacteriology found 2 strains of Klebsiella penumoniae subsp. pneumoniae
from liver and leg with necrosis. Both strains were biochemically identical.
Morphological and biochemical properties of the strains:
On blood agar: S-type colonies, nonhemolytic, 2-3 mm in diameter.
On MacConkey agar: lactose positive colonies
On Mueller-Hinton agar: nonpigmented, smooth colonies.
Aerobic, facultatively anaerobic, Gram-negative, nonmotile rods.
Catalase positive and oxidase negative.
TSI, MIU and Simmons results:
TSI - glucose, lactose and sacharose were fermented.
Gas was produced from glucose. H2S was not produced.
MIU - indole, urea and motility negative
Simmons - citrate positive
Biochemical results on API 20 E strip:
ONPG, citrate, urea, Voges-Proskauer, glucose,
mannose, inositol, sorbitol, rhamnose, sacharose,
melibiose, amylase, arabinose positive. Urea became
positive after 48 hours.
Nitrates rwere educed.
Arginine dihydrolase, lysine decarboxylase, ornithine
decarboxylase, H2S production, TDA, indole production
and gelatin liquefaction were negative.
TSI - glucose, lactose and sacharose were fermented.
Gas was produced from glucose. H2S was not produced.
MIU - indole, urea and motility negative
Simmons - citrate positive
Biochemical results on API 20 E strip:
ONPG, citrate, urea, Voges-Proskauer, glucose,
mannose, inositol, sorbitol, rhamnose, sacharose,
melibiose, amylase, arabinose positive. Urea became
positive after 48 hours.
Nitrates rwere educed.
Arginine dihydrolase, lysine decarboxylase, ornithine
decarboxylase, H2S production, TDA, indole production
and gelatin liquefaction were negative.
API identified strain as Klebsiella penumoniae subsp. pneumoniae 98%
With ABIS 6 online software result was Klebsiella penumoniae subsp. pneumoniae 91% (with a question
mark on the negative Lysine decarboxylase). Next choice in both identification systems was Klebsiella
planticola.
Both isolated strains were sensible to Colistin sulphate, intermediate to Gentamicin, Neomycin and resistant
to Enrofloxacin, Streptomycin, Kanamycin, Lincomycin, Amoxicillin, Bacitracin,
Sulphamethoxazole+Trimethoprim, Ampicillin, Trimethoprim, Spectinomycin and Tetracycline.
With ABIS 6 online software result was Klebsiella penumoniae subsp. pneumoniae 91% (with a question
mark on the negative Lysine decarboxylase). Next choice in both identification systems was Klebsiella
planticola.
Both isolated strains were sensible to Colistin sulphate, intermediate to Gentamicin, Neomycin and resistant
to Enrofloxacin, Streptomycin, Kanamycin, Lincomycin, Amoxicillin, Bacitracin,
Sulphamethoxazole+Trimethoprim, Ampicillin, Trimethoprim, Spectinomycin and Tetracycline.
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