| MIU (Motility Indole Urea) |
DESCRIPTION
Test checks simultaneously for the production of indole, urea hydrolysis and bacteria motility. Medium, together with TSI and Simmons
agar, is most used for Enterobacteriaceae identification, especially Escherichia and Salmonella.
Test checks simultaneously for the production of indole, urea hydrolysis and bacteria motility. Medium, together with TSI and Simmons
agar, is most used for Enterobacteriaceae identification, especially Escherichia and Salmonella.
MEDIUM PREPARATION
Composition:
MIU base
Proteose peptone Difco 10g, beef extract 5g, NaCl 5g, KH2PO4 2g, phenol red
solution 1/500 6 ml, agar 3g, distilled water 900ml.
Adjust pH to 6.8-6.9
Urea - glucose solution
10 g urea + 1 g glucose + distilled water ad 100 ml.
Preparation: to 90 ml base medium add 10 ml urea-glucose solution. Other
commercial MIU base media include glucose, and only require 10 ml sol. 40% urea
to be added.
PROCEDURE
Harvest a well isolated colony and inoculate a MIU tube by stabbing the medium.
Incubate at 37 °C, 24 hours.
RESULTS
Motility is positive if the entire medium became opaque (semisolid state of the
medium permits the bacteria moving). Result is negative if the culture grows only on
the stabbing line.
For the indole test add 2-3 drops of Ehrlich-Covacs reactive. A red layer on the
surface of the medium appears if the reaction is positive.
Urea is hydrolysed if the entire medium turns red.
Composition:
MIU base
Proteose peptone Difco 10g, beef extract 5g, NaCl 5g, KH2PO4 2g, phenol red
solution 1/500 6 ml, agar 3g, distilled water 900ml.
Adjust pH to 6.8-6.9
Urea - glucose solution
10 g urea + 1 g glucose + distilled water ad 100 ml.
Preparation: to 90 ml base medium add 10 ml urea-glucose solution. Other
commercial MIU base media include glucose, and only require 10 ml sol. 40% urea
to be added.
PROCEDURE
Harvest a well isolated colony and inoculate a MIU tube by stabbing the medium.
Incubate at 37 °C, 24 hours.
RESULTS
Motility is positive if the entire medium became opaque (semisolid state of the
medium permits the bacteria moving). Result is negative if the culture grows only on
the stabbing line.
For the indole test add 2-3 drops of Ehrlich-Covacs reactive. A red layer on the
surface of the medium appears if the reaction is positive.
Urea is hydrolysed if the entire medium turns red.
NOTES
Melt base medium by boiling it and wait to cool under 50 ºC before adding Urea -
glucose solution, otherwise urea will be inactivated. Final medium state must be
semisolid.
Melt base medium by boiling it and wait to cool under 50 ºC before adding Urea -
glucose solution, otherwise urea will be inactivated. Final medium state must be
semisolid.
REFERENCES:
1. Helgomar Raducanescu, Valeria.Bica-Popii,1986. Bacteriologie veterinara, Ed.
Ceres, Bucuresti.
1. Helgomar Raducanescu, Valeria.Bica-Popii,1986. Bacteriologie veterinara, Ed.
Ceres, Bucuresti.
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(c) Costin Stoica
A demonstration of TSI, MIU and Simmons utilization is available in video section.
| R E G N U M PROKARYOTAE |
| Back |
| Indole-positive result: red ring on MIU medium surface (middle tube) |
| Left: non-motile bacteria - growth on stabbing line. Right: motile bacteria - uniform medium turbidity. |
| Urea-positive result: MIU medium turning red (middle tube) |
