| Pseudomonas leptonychotis |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas leptonychotis Novakova et al. 2020.
Genus Pseudomonas, Pseudomonas leptonychotis Novakova et al. 2020.
Gram-negative, slim rods, 0.5-0.7 x 2-4 µm, occurring mainly in groups or singly.
Longer cells may be curved. Motile by one or more polar flagella.
Longer cells may be curved. Motile by one or more polar flagella.
Colonies are yellowish, circular with slightly irregular margins, smooth and shiny,
reaching about 2 mm in diameter when cultivated on TSA at 30 ºC for 24 h. Older
cultures get sticky. Grows well in the temperature range 2-35 ºC (optimally at 25 ºC),
in the presence of 0.5–3% NaCl (optimally in 1% NaCl) and at a pH ranging from 6 to
8 (optimum pH 7). No fluorescein pigment is observed on King B medium. Grow well
on PCA, TSA, nutrient agar CM3, BHI agar, MacConkey agar, as well as on Columbia
agar supplemented with 5% sheep blood without haemolysis; no or weak growth is
observed on R2A agar.
reaching about 2 mm in diameter when cultivated on TSA at 30 ºC for 24 h. Older
cultures get sticky. Grows well in the temperature range 2-35 ºC (optimally at 25 ºC),
in the presence of 0.5–3% NaCl (optimally in 1% NaCl) and at a pH ranging from 6 to
8 (optimum pH 7). No fluorescein pigment is observed on King B medium. Grow well
on PCA, TSA, nutrient agar CM3, BHI agar, MacConkey agar, as well as on Columbia
agar supplemented with 5% sheep blood without haemolysis; no or weak growth is
observed on R2A agar.
Isolated from anus or mouth samples of Weddell seals (Leptonychotes weddellii) at James Ross Island, Antarctica.
Sensitive to carbenicillin, ceftazidim, ciprof loxacin, gentamicin, imipenem, kanamycin, cotrimoxazole, piperacillin, polymyxin B,
streptomycin and tetracycline. Resistant to ampicillin, cefixime, cephalothin and chloramphenicol.
Sensitive to carbenicillin, ceftazidim, ciprof loxacin, gentamicin, imipenem, kanamycin, cotrimoxazole, piperacillin, polymyxin B,
streptomycin and tetracycline. Resistant to ampicillin, cefixime, cephalothin and chloramphenicol.
Undetermined.
- Novakova D, Svec P, Zeman M, Busse HJ, Maslanova I, Pantucek R, Kralova S, Kristofova L, Sedlacek I. Pseudomonas leptonychotis
sp. nov., isolated from Weddell seals in Antarctica. Int J Syst Evol Microbiol 2020; 70:302-308.
Positive results for acid and alkaline phosphatase, catalase, Simmons citrate, casein hydrolysis, esterase (C4), esterase lipase (C8),
lipase (C14), leucine arylamidase, lecithine hydrolysis, oxidase, tyrosine hydrolysis, Tween 80 hydrolysis, valine arylamidase (weak),
acid production from mannose and maltose.
Can utilize (Biolog GEN III ): D-fructose-6-phosphate, D-serine, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, L-histidine,
methyl pyruvate, L-lactic acid, citric acid, alpha-keto-glutaric acid (weak), D- and L-malic acid, bromo-succinic acid, Tween 40,
gamma-aminobutyric acid, beta-hydroxy-D,L-butyric acid, acetoacetic acid, propionic acid and acetic acid.
Negative results for production of levane from sucrose, cystine arylamidase, DNase, aesculin hydrolysis, alpha-fucosidase, alpha-
and beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase,
nitrite reduction, naphtol-AS-BI-phosphohydrolase, starch hydrolysis, trypsin, alpha-chymotrypsin and urease.
No utilization of: acetamide utilization, sodium malonate, maltose, trehalose, cellobiose, sucrose, turanose, stachyose, raffinose,
lactose, methyl beta-D-glucoside, D-salicin, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine,
N-acetyl neuraminic acid, alpha-D-glucose, D-mannose, D-galactose, 3-methyl glucose, inosine, D-sorbitol, D-mannitol, D-arabitol,
myo-inositol, glycerol, D-glucose-6-phosphate , D-aspartic acid, gelatin, glycyl-L-proline, L-pyroglutamic acid, L-serine, D-gluconic
acid, quinic acid, D-saccharic acid, p-hydroxy phenylacetic acid, D-lactic acid methyl ester and formic acid.
Variable results for gelatinase, nitrate reduction, acid production from fructose and xylose.
Variable utilization of: dextrin, D- and L-fucose, D-glucuronic, mucic acid, gentiobiose, melibiose, D-fructose, L-rhamnose, pectin,
D-galacturonic acid, D-galactonic acid lactone, glucuronamide, alpha-hydroxy-butyric acid and alpha-keto butyric acid.
lipase (C14), leucine arylamidase, lecithine hydrolysis, oxidase, tyrosine hydrolysis, Tween 80 hydrolysis, valine arylamidase (weak),
acid production from mannose and maltose.
Can utilize (Biolog GEN III ): D-fructose-6-phosphate, D-serine, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, L-histidine,
methyl pyruvate, L-lactic acid, citric acid, alpha-keto-glutaric acid (weak), D- and L-malic acid, bromo-succinic acid, Tween 40,
gamma-aminobutyric acid, beta-hydroxy-D,L-butyric acid, acetoacetic acid, propionic acid and acetic acid.
Negative results for production of levane from sucrose, cystine arylamidase, DNase, aesculin hydrolysis, alpha-fucosidase, alpha-
and beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase,
nitrite reduction, naphtol-AS-BI-phosphohydrolase, starch hydrolysis, trypsin, alpha-chymotrypsin and urease.
No utilization of: acetamide utilization, sodium malonate, maltose, trehalose, cellobiose, sucrose, turanose, stachyose, raffinose,
lactose, methyl beta-D-glucoside, D-salicin, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine,
N-acetyl neuraminic acid, alpha-D-glucose, D-mannose, D-galactose, 3-methyl glucose, inosine, D-sorbitol, D-mannitol, D-arabitol,
myo-inositol, glycerol, D-glucose-6-phosphate , D-aspartic acid, gelatin, glycyl-L-proline, L-pyroglutamic acid, L-serine, D-gluconic
acid, quinic acid, D-saccharic acid, p-hydroxy phenylacetic acid, D-lactic acid methyl ester and formic acid.
Variable results for gelatinase, nitrate reduction, acid production from fructose and xylose.
Variable utilization of: dextrin, D- and L-fucose, D-glucuronic, mucic acid, gentiobiose, melibiose, D-fructose, L-rhamnose, pectin,
D-galacturonic acid, D-galactonic acid lactone, glucuronamide, alpha-hydroxy-butyric acid and alpha-keto butyric acid.
(c) Costin Stoica
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