| Pseudomonas karstica |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas karstica Svec et al. 2020.
Species description is based on a single isolate.
Genus Pseudomonas, Pseudomonas karstica Svec et al. 2020.
Species description is based on a single isolate.
Gram-negative, short rods. Motile by single polar flagellum.
Colonies on TSA medium are smooth with regular margins and 1-2mm in diameter
when incubated at 20 ºC for 48h. Produces fluorescent pigment on King B medium.
Growth occurs in the temperature range of 0-30 ºC, in the presence of 0.5-3% NaCl
and at a pH ranging from pH 4 to 9.Grows well on plate count agar, Reasoner's 2A
agar, nutrient agar CM3, brain heart infusion agar, MacConkey agar and on Columbia
agar supplemented with 5% sheep blood with haemolysis.
when incubated at 20 ºC for 48h. Produces fluorescent pigment on King B medium.
Growth occurs in the temperature range of 0-30 ºC, in the presence of 0.5-3% NaCl
and at a pH ranging from pH 4 to 9.Grows well on plate count agar, Reasoner's 2A
agar, nutrient agar CM3, brain heart infusion agar, MacConkey agar and on Columbia
agar supplemented with 5% sheep blood with haemolysis.
Isolated from a moonmilk sample obtained from the wall of the ‘Horni v chobotu’ cave (The Moravian Karst, Czech Republic).
Undetermined.
- Svec P, Kosina M, Zeman M, Holochova P, Kralova S, Nemcova E, Micenkova L, Urvashi, Gupta V, Sood U, et al. Pseudomonas
karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves. Int J Syst Evol Microbiol
2020; 70:5131-5140.
Positive results for acid phosphatase, alkaline phosphatase (weak), arginine dihydrolase, catalase, casein hydrolysis, Simmons citrate
and sodium malonate utilization, esterase lipase (C8), esterase (C4) (weak), leucine arylamidase, lecithinase (weak),
naphthol-AS-BI-phosphohydrolase (weak), tyrosine hydrolysis, oxidase, valine arylamidase (weak), acid production (oxidative, not
fermentative) from: fructose, glucose,mannitol and xylose.
Can utilize (Biolog GEN III): alpha-D-glucose, D-galactose, D-fucose, L-rhamnose, D- and L-aspartic acid, L-glutamic acid, L-serine,
D-galacturonic acid, L-galactonic acid lactone, D-gluconic acid, D-glucuronic acid, glucuronamide, mucic acid, citric acid, L-malic acid,
Tween 40 and acetic acid.
Negative results for cystine arylamidase, DNase, aesculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase, alpha- and
beta-glucosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase, gelatin hydrolysis, H2S production, lysine decarboxylase, lipase
(C14), alpha-mannosidase, nitrate and nitrite reduction, ornithine decarboxylase, ONPG, starch hydrolysis, Tween 80, urease, trypsin,
alpha-chymotrypsin, acid production from maltose.
No utilization of acetamide, dextrin, maltose, cellobiose, gentiobiose, sucrose, turanose, stachyose, raffinose, lactose, melibiose,
methyl beta-D-glucoside, D-salicin, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine,
N-acetyl neuraminic acid, 3-methyl glucose, L-fucose, D-sorbitol, D-glucose-6-phosphate, gelatin, glycyl-L-proline, L-arginine,
L-pyroglutamic acid, pectin, quinic acid, p-hydroxy-phenylacetic acid, methyl pyruvate, D-lactic acid methyl ester, alpa-keto-glutaric acid,
D-malic acid, bromosuccinic acid, alpha-hydroxy-butyric acid, beta-hydroxy-D,L-butyric acid, alpha-keto-butyric acid, acetoacetic acid,
propionic acid and formic acid.
Variable results for trehalose, D-mannose, D-fructose, inosine, D-mannitol, D-arabitol, myo-inositol, glycerol, D-fructose-6-phosphate,
D-serine, L-alanine, L-histidine, D-saccharic acid, L-lactic acid and gamma-amino-butyric acid.
and sodium malonate utilization, esterase lipase (C8), esterase (C4) (weak), leucine arylamidase, lecithinase (weak),
naphthol-AS-BI-phosphohydrolase (weak), tyrosine hydrolysis, oxidase, valine arylamidase (weak), acid production (oxidative, not
fermentative) from: fructose, glucose,mannitol and xylose.
Can utilize (Biolog GEN III): alpha-D-glucose, D-galactose, D-fucose, L-rhamnose, D- and L-aspartic acid, L-glutamic acid, L-serine,
D-galacturonic acid, L-galactonic acid lactone, D-gluconic acid, D-glucuronic acid, glucuronamide, mucic acid, citric acid, L-malic acid,
Tween 40 and acetic acid.
Negative results for cystine arylamidase, DNase, aesculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase, alpha- and
beta-glucosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase, gelatin hydrolysis, H2S production, lysine decarboxylase, lipase
(C14), alpha-mannosidase, nitrate and nitrite reduction, ornithine decarboxylase, ONPG, starch hydrolysis, Tween 80, urease, trypsin,
alpha-chymotrypsin, acid production from maltose.
No utilization of acetamide, dextrin, maltose, cellobiose, gentiobiose, sucrose, turanose, stachyose, raffinose, lactose, melibiose,
methyl beta-D-glucoside, D-salicin, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine,
N-acetyl neuraminic acid, 3-methyl glucose, L-fucose, D-sorbitol, D-glucose-6-phosphate, gelatin, glycyl-L-proline, L-arginine,
L-pyroglutamic acid, pectin, quinic acid, p-hydroxy-phenylacetic acid, methyl pyruvate, D-lactic acid methyl ester, alpa-keto-glutaric acid,
D-malic acid, bromosuccinic acid, alpha-hydroxy-butyric acid, beta-hydroxy-D,L-butyric acid, alpha-keto-butyric acid, acetoacetic acid,
propionic acid and formic acid.
Variable results for trehalose, D-mannose, D-fructose, inosine, D-mannitol, D-arabitol, myo-inositol, glycerol, D-fructose-6-phosphate,
D-serine, L-alanine, L-histidine, D-saccharic acid, L-lactic acid and gamma-amino-butyric acid.
(c) Costin Stoica
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