| Pseudomonas brassicae |
Positive results for arginine dihydrolase (negative in API 20 NE), gelatin hydrolysis (negative in API 20 NE) and oxidase.
Can utilize (Biolog GEN III MicroPlate system - 4 days incubation at 27 ºC): alpha-D-glucose, D-fructose, inosine, glycerol, L-alanine, L-
arginine, L-aspartic acid, L-glutamic acid, L-histidine, L-serine, D-galacturonic acid, L-galactonic acid lactone, D-gluconic acid, quinic
acid, p‐hydroxyphenylacetic acid, methyl pyruvate, L-lactic acid, citric acid, alpha-ketoglutaric acid, D- and L-malic acid, bromosuccinic
acid, gamma-aminobutyric acid, alpha-hydroxybutyric acid, DL-beta-hydroxybutyric acid, alpha-ketobutyric acid, propionic acid, acetic
acid and formic acid.
Can utilize (API 20 NE): malate, citrate and phenylacetate.
Negative results for levan formation from sucrose, 2-ketogluconate production, margarine hydrolysis, acid production from sucrose,
nitrate reduction, indole production, glucose fermentation, urease, aesculin hydrolysis and beta-galactosidase.
No utilization of: dextrin, maltose, trehalose, cellobiose, gentiobiose, sucrose, turanose, stachyose, D-raffinose, alpha-D-lactose,
melibiose, methyl beta-D-glucoside, D-salicin, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine,
N-acetyl neuraminic acid, D-mannose, D-galactose, 3-methyl glucose, L-rhamnose, D-sorbitol, D-mannitol, D-arabitol, myo-inositol,
D-glucose-6-phosphate, D-aspartic acid, D-serine, gelatin, glycyl-lproline, L-pyroglutamic acid, mucic acid, D-saccharic acid, D-lactic
acid methyl ester and Tween 40.
No utilization (API 20 NE): caprate, arabinose, mannose, mannitol, N-acetylglucosamine, maltose and adipate.
Contradictory results for the utilization of glucose and gluconate.
Can utilize (Biolog GEN III MicroPlate system - 4 days incubation at 27 ºC): alpha-D-glucose, D-fructose, inosine, glycerol, L-alanine, L-
arginine, L-aspartic acid, L-glutamic acid, L-histidine, L-serine, D-galacturonic acid, L-galactonic acid lactone, D-gluconic acid, quinic
acid, p‐hydroxyphenylacetic acid, methyl pyruvate, L-lactic acid, citric acid, alpha-ketoglutaric acid, D- and L-malic acid, bromosuccinic
acid, gamma-aminobutyric acid, alpha-hydroxybutyric acid, DL-beta-hydroxybutyric acid, alpha-ketobutyric acid, propionic acid, acetic
acid and formic acid.
Can utilize (API 20 NE): malate, citrate and phenylacetate.
Negative results for levan formation from sucrose, 2-ketogluconate production, margarine hydrolysis, acid production from sucrose,
nitrate reduction, indole production, glucose fermentation, urease, aesculin hydrolysis and beta-galactosidase.
No utilization of: dextrin, maltose, trehalose, cellobiose, gentiobiose, sucrose, turanose, stachyose, D-raffinose, alpha-D-lactose,
melibiose, methyl beta-D-glucoside, D-salicin, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine,
N-acetyl neuraminic acid, D-mannose, D-galactose, 3-methyl glucose, L-rhamnose, D-sorbitol, D-mannitol, D-arabitol, myo-inositol,
D-glucose-6-phosphate, D-aspartic acid, D-serine, gelatin, glycyl-lproline, L-pyroglutamic acid, mucic acid, D-saccharic acid, D-lactic
acid methyl ester and Tween 40.
No utilization (API 20 NE): caprate, arabinose, mannose, mannitol, N-acetylglucosamine, maltose and adipate.
Contradictory results for the utilization of glucose and gluconate.
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas brassicae Sawada et al. 2020.
Genus Pseudomonas, Pseudomonas brassicae Sawada et al. 2020.
Gram-negative rods, 0.9-1.3 x 1.6-3.0 µm. Motile by one or two polar flagella.
Non-spore-forming.
Non-spore-forming.
Colonies on nutrient agar plates are pale yellow, opaque, round with entire to
somewhat undulate margins, raised, smooth, glistening and usually 1-2 mm in
diameter after 2 days incubation at 27 ºC. No water-soluble pigments are produced.
Growth occurs at 4-35 ºC but not at 37 ºC. Produce fluorescent pigment on King’s B
medium. Aerobic.
somewhat undulate margins, raised, smooth, glistening and usually 1-2 mm in
diameter after 2 days incubation at 27 ºC. No water-soluble pigments are produced.
Growth occurs at 4-35 ºC but not at 37 ºC. Produce fluorescent pigment on King’s B
medium. Aerobic.
Isolated from a head rot lesion of broccoli (Brassica oleracea L. var. italica Plenck) in Hokkaido, Japan.
Pathogenic to broccoli heads.
Experimental infection: positive for potato soft rot; negative for tobacco hypersensitive reaction.
Experimental infection: positive for potato soft rot; negative for tobacco hypersensitive reaction.
- Sawada H, Fujikawa T, Horita H. Pseudomonas brassicae sp. nov., a pathogen causing head rot of broccoli in Japan. Int J Syst
Evol Microbiol 2020; 70:5319-5329. - Wright MH, Hanna JG, Pica D, Tebo BM. Pseudomonas laurentiana sp. nov., an Mn(III)-oxidizing bacterium isolated from the St.
Lawrence Estuary. Pharmacogn Commn 2018; 8:153-157.
(c) Costin Stoica
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