| Pseudomonas asplenii |
Positive results for gelatin liquefaction, arginine dihydrolase, acid (without gas) production from glucose, galactose, fructose,
arabinose, xylose, maltose, sucrose, lactose (weak, after long incubation).
Can assimilate: glycerol, D-glucose, D-fructose and gluconate.
Can utilize: malonic acid, D,L-lactic acid, D-arabitol, methyl pyruvate, cis-aconitic acid, citric acid, formic acid, beta-hydroxybutyric acid,
p-hydroxyphenylacetic acid, alpha-ketoglutaric acid, quinic acid, bromosuccinic acid, D-alanine, L-aspartic acid, L-glutamic acid,
L-histidine, hydroxy-L-proline, L-proline, L-serine, DL-carnitine, gamma-aminobutyric acid and urocanic acid.
Negative results for lecithinase, indole production, levan formation, nitrates reduction, hydrogen sulfide, urease, lecithinase, esculin
hydrolysis and starch hydrolysis (former P. fuscovaginae strains can hydrolyse starch).
No assimilation of: itaconate, L-leucine, erythritol, D-arabinose, L-xylose, adonitol, methyl beta-xyloside, L-sorbose, L-rhamnose,
dulcitol, inositol, D-sorbitol, methyl alpha- D-mannoside, methyl alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin,
D-cellobiose, D-maltose, D-lactose, D-melibiose, D-sucrose, inulin, D-melezitose, D-raffinose, starch, glycogen, xylitol, gentiobiose,
D-turanose, D-lyxose, D-tagatose, D-fucose, L-fucose, L-arabitol, 2- and 5-ketogluconate.
No utilization of: dextrin, glycogen, Tween 40, N-acetyl-D-galactosamine, adonitol, D-cellobiose, erythritol, L-fucose, gentiobiose,
myo-inositol, lactose, lactulose, maltose, D-melibiose, methyl beta-D-glucoside, D-psicose, D-raffinose, L-rhamnose, D-sorbitol,
sucrose, D-trehalose, turanose and xylitol.
arabinose, xylose, maltose, sucrose, lactose (weak, after long incubation).
Can assimilate: glycerol, D-glucose, D-fructose and gluconate.
Can utilize: malonic acid, D,L-lactic acid, D-arabitol, methyl pyruvate, cis-aconitic acid, citric acid, formic acid, beta-hydroxybutyric acid,
p-hydroxyphenylacetic acid, alpha-ketoglutaric acid, quinic acid, bromosuccinic acid, D-alanine, L-aspartic acid, L-glutamic acid,
L-histidine, hydroxy-L-proline, L-proline, L-serine, DL-carnitine, gamma-aminobutyric acid and urocanic acid.
Negative results for lecithinase, indole production, levan formation, nitrates reduction, hydrogen sulfide, urease, lecithinase, esculin
hydrolysis and starch hydrolysis (former P. fuscovaginae strains can hydrolyse starch).
No assimilation of: itaconate, L-leucine, erythritol, D-arabinose, L-xylose, adonitol, methyl beta-xyloside, L-sorbose, L-rhamnose,
dulcitol, inositol, D-sorbitol, methyl alpha- D-mannoside, methyl alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin,
D-cellobiose, D-maltose, D-lactose, D-melibiose, D-sucrose, inulin, D-melezitose, D-raffinose, starch, glycogen, xylitol, gentiobiose,
D-turanose, D-lyxose, D-tagatose, D-fucose, L-fucose, L-arabitol, 2- and 5-ketogluconate.
No utilization of: dextrin, glycogen, Tween 40, N-acetyl-D-galactosamine, adonitol, D-cellobiose, erythritol, L-fucose, gentiobiose,
myo-inositol, lactose, lactulose, maltose, D-melibiose, methyl beta-D-glucoside, D-psicose, D-raffinose, L-rhamnose, D-sorbitol,
sucrose, D-trehalose, turanose and xylitol.
Taxonomy
Morphology
Growth conditions
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas asplenii (Phytomonas asplenii Ark and Tompkins 1946) Savulescu 1947.
According toTohya et al. 2020 Pseudomonas fuscovaginae is a later heterotypic synonym of Pseudomonas asplenii.
Genus Pseudomonas, Pseudomonas asplenii (Phytomonas asplenii Ark and Tompkins 1946) Savulescu 1947.
According toTohya et al. 2020 Pseudomonas fuscovaginae is a later heterotypic synonym of Pseudomonas asplenii.
Gram-negative, 0.3-0.5 x 1.2-2.4 μm, rods. Motile, with 1 to 3 polar flagella.
Colonies on nutrient agar are circular, smooth and 2–3 mm in diameter (after 24 h of
cultivation). Fluorescein is produced on King B medium. Pyocyanine is not produced
on King A medium. Strictly aerobic, optimum growth temperature 26 ºC, minimum 1
ºC, maximum 34 ºC.
Grows on media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar / Nutrient
broth, Fermi's, Cohn's and Uschinsky's solutions
cultivation). Fluorescein is produced on King B medium. Pyocyanine is not produced
on King A medium. Strictly aerobic, optimum growth temperature 26 ºC, minimum 1
ºC, maximum 34 ºC.
Grows on media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar / Nutrient
broth, Fermi's, Cohn's and Uschinsky's solutions
Isolated from soil and plants (ferns - Asplenium nidus). Former P. fuscovaginae type strain was isolated from diseased leaf sheaths
of Oryza sativa in Japan.
of Oryza sativa in Japan.
Fern pathogen - causes bacterial leaf blight of Asplenium nidus.
P. fuscovaginae is pathogenic for the Gramineae. Is the causative agent of sheath brown rot of rice. Pathogenicity was
demonstrated on Oryza sativa, Hordeum vulgare, Triticum aestivum, Avena sativa, Zea mays, Lolium perenne, Bromus marginatus,
Phleum pratense and Phalaris arundinacea. Produce a hypersensitivity reaction in tobacco plants.
P. fuscovaginae is pathogenic for the Gramineae. Is the causative agent of sheath brown rot of rice. Pathogenicity was
demonstrated on Oryza sativa, Hordeum vulgare, Triticum aestivum, Avena sativa, Zea mays, Lolium perenne, Bromus marginatus,
Phleum pratense and Phalaris arundinacea. Produce a hypersensitivity reaction in tobacco plants.
- Ark P.A. & Tompkins, CM (1946). "Bacterial leaf blight of bird's-nest fern". Phytopathology 36: 758-761.
- Savulescu T.: Contribution à la classification des bacteriacées phytopathogènes. Analele Academiei Romane Series III Tom 22,
Memoire, 1947, 4, 1-26. - Tvrzova L., Schumann P., Sproer C., Sedlacek I., Pacova Z., Sedo O., Zdrahal Z., Steffen M. & Lang E.: Pseudomonas moraviensis
sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds & emended description of
Pseudomonas asplenii. Int. J. Syst. Evol. Microbiol., 2006, 56, 2657-2663. - Wang LT, Tai CJ, Wu YC, Chen YB, Lee FL, Wang SL. Pseudomonas taiwanensis sp. nov., isolated from soil. Int J Syst Evol
Microbiol 2010; 60:2094-2098. - Tohya et al. 2020 Tohya M, Watanabe S, Tada T, Tin HH, Kirikae T. Genome analysis-based reclassification of Pseudomonas
fuscovaginae and Pseudomonas shirazica as later heterotypic synonyms of Pseudomonas asplenii and Pseudomonas asiatica,
respectively. Int J Syst Evol Microbiol 2020; 70:3547-3552.
(c) Costin Stoica
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