Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, [Group B lactobacilli
(facultatively heterofermentative), unique phylogenetic-group], Lactobacillus graminis Beck, Weiss and Winter 1989.
Gram-positive, slightly curved rods with rounded ends, 0.7-1.0 x 1.5-2 µm, occurring
singly, in pairs, or short chains. Nonmotile.
Colonies are smooth, round, nonpigmented. Produce a flocculant sediment after 3
days of growth in MRS broth. Can grow at 15 ºC, but not at 45 ºC.
Habitat: grass silage.
- Hammes W.P. and Hertel C., 2009. Genus I. Lactobacillus Beijerinck 1901. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
Facultatively heterofermentative (hexoses are fermented almost exclusively to lactic
acid by the Embden-Meyerhof pathway or, at least by some species, to lactic acid,
acetic acid, ethanol, and formic acid under glucose limitations; pentoses are fermented to lactic acid and acetic acid via an inducible
Positive results for fermentation of: amygdalin, cellobiose, esculin, sucrose, and D-xylose.
Negative results for fermentation of: arabinose, dulcitol, gluconate, mannitol, melezitose, melibiose, raffinose, ribose, and sorbitol.
(c) Costin Stoica