Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Actinomycinae, Family
Actinomycetaceae, Genus Actinomyces, Actinomyces graevenitzii Pascual Ramos, Falsen , Alvarez, Akervall, Sjöden, and Collins
Gram-positive straight or slightly curved rods with some branching and swollen ends.
Colonies are opaque, nonpigmented, 0.2 mm in diameter on Columbia agar (Difco)
supplemented with 5% horse blood after 24 h at 37 ºC in air plus 5% CO2. Colonies
adhere to agar surfaces. Facultative anaerobic.
Isolated from human clinical specimens (mainly respiratory tract secretions). Habitat is not known.
Isolated from clinical specimens (bronchus brush, bronchial secretion, sputum and from jaw of a patient with osteitis).
- Pascual Ramos C., Falsen E., Alvarez N., Akervall E., Sjöden B., and Collins M.D., 1997. Actinomyces graevenitzii sp. nov.,
isolated from human clinical specimens. Int. J. Syst. Bacteriol. Vol. 47, No. 3, 885-888.
Description is based mostly on API CORYNE, API ZYM, API 50CH (with API 50 CHE
medium) and Rapid ID 32A results.
Positive results for acid phosphatase, beta-galactosidase, N-acetyl-beta-glucosaminidase, leucine arylamidase, phosphoamidase,
acid production from: fructose, galactose, glycerol, N-acetylglucosamine, inositol, 5 ketogluconate, lactose, maltose, ribose, sucrose
(saccharose) & tagatose.
Negative results for catalase, chymotrypsin, esculin hydrolysis, alpha-fucosidase, alpha-galactosidase, alpha- and
beta-glucosidase,beta-glucuronidase, hippurate hydrolysis, lipase C14, alpha-mannosidase, nitrate reduction, pyrrolidonyl
arylamidase, urease, valine arylamidase, acid production from: D-arabinose, amygdalin, D- and L-arabitol, cellobiose, dulcitol,
erythritol, D- and L-fucose, glycogen, gentibiose, methyl-beta-D-glucopyranoside, inulin, mannitol, melezitose, melibiose, rhamnose,
salicin, sorbitol, L-sorbose, starch, trehalose, xylitol, L- and D-xylose.
Variable results for acid production from adonitol & mannose.
(c) Costin Stoica