Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Actinomycinae, Family
Actinomycetaceae, Genus Actinobaculum, Actinobaculum massiliense Greub and Raoult 2006.
Historical synonym: Actinobaculum massiliae Greub and Raoult 2002.
Gram-positive straight to slightly curved rods, some of which exhibit branching.
Non-motile. Non-acid-fast. Non-spore-forming.
On sheep blood Columbia agar, colonies are 1.0 mm in diameter after 3 days of
incubation at 37 ºC in a 5% CO2 atmosphere. Non-haemolytic. CAMP reaction is
negative. Facultative anaerobic.
Isolated from urine.
- Yassin A.F., Sproer C., Pukall R., Sylvester M., Siering C. and Schumann P., 2015. Dissection of the genus Actinobaculum:
reclassification of Actinobaculum schaalii Lawson et al. 1997 and Actinobaculum urinale Hall et al. 2003 as Actinotignum schaalii
gen. nov., comb. nov. and Actinotignum urinale comb. nov., description of Actinotignum sanguinis sp. nov. and emended
descriptions of the genus Actinobaculum and Actinobaculum suis; and re-examination of the culture deposited as Actinobaculum
massiliense CCUG 47753T (5DSM 19118T), revealing that it does not represent a strain of this species. J Syst Evol Microbiol 65,
- Greub G. and Raoult D., 2002. “Actinobaculum massiliae”, a new species causing chronic urinary tract infection. J Clin Microbiol
40, 11, 3938-3941.
Description is based on API Coryne and API 20 Strep results.
Positive results for alpha-glucosidase, hippurate hydrolysis, leucine arylamidase, pyrazinamidase, acid production from D-glucose,
maltose, ribose, trehalose & D-xylose.
Negative results for acid and alkaline phosphatase, arginine dihydrolase, catalase, cystine arylamidase, esculin hydrolysis, esterase
(C4), esterase lipase (C8), alpha-fucosidase, alpha- and beta-galactosidase, gelatin hydrolysis, beta-glucuronidase,
N-acetyl-beta-glucosaminidase, lipase (C14), alpha-mannosidase, nitrate reduction, oxidase, pyrrolidonyl arylamidase, trypsin,
alpha-chymotrypsin, urease, valine arylamidase, Voges-Proskauer test, acid production from L-arabinose, glycogen, inulin, lactose,
mannitol, mannose, raffinose, sorbitol, sucrose and starch.
(c) Costin Stoica
Isolated from the urine of women with cystitis.