Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Achromobacter,
Achromobacter agilis Vandamme et al. 2016.
Old synnym: Bacillus denitrificans agilis Ampola and Garino 1896.
Gram-negative rods, 0.4-0.7 x 0.9-1.5 µm. Motile.
Colonies are low convex, translucent and non-pigmented, with smooth margins and
1-1.5 mm in diameter after 48h incubation on trypticase soy agar at 28 ºC. Grows on,
blood agar, Drigalsky agar, cetrimide agar and on acetamide. Nonhaemolytic. Can
grow in 3% NaCl, but not in 4.5% NaCl. No growth at 42 ºC. No fluorescence on King’
s B medium.
Isolated from cow manure.
- Vandamme, P. A., Peeters, C., Inganas, E., Cnockaert, M., Houf, K., Spilker, T., Moore, E. R. B. and Lipuma, J. J. 2016. Taxonomic
dissection of Achromobacter denitrificans Coenye et al. 2003 and proposal of Achromobacter agilis sp. nov., nom. rev.,
Achromobacter pestifer sp. nov., nom. rev., Achromobacter kerstersii sp. nov. and Achromobacter deleyi sp. nov. Int. J. Syst. Evol.
Microbiol., 66, 3708-3717.
Positive results for catalase, C4 lipase, nitrate and nitrite reduction, denitrification, oxidase.
Assimilate adipate (positive in API 20 NE test, but negative by classical test).
Negative results for acid and alkaline phosphatase, amylase, arginine dihydrolase, esculin hydrolysis, gelatin hydrolysis,
beta-glucosidase, DNase, H2S production, indole production, lysine decarboxylase, ornithine decarboxylase, urease, ONPG
beta-galactosidase, phosphoamidase, acid production from glucose.
No assimilation of: trehalose, L-arginine, DL-norleucine, D-mannitol, sucrose, adonitol, D-xylose, D-fructose, L-arabinose,
D-mannose, N-acetylglucosamine, maltose, D-gluconate, caprate, L-malate, citrate, phenylacetate, D-lactate, lactate + methionine &
(c) Costin Stoica