PROCEDURE
Disperse culture or pathologic material on the medium surface, then incubate 48 hours
at 37 ºC.
RESULTS
Salmonella strains (excepting S. paratyphi A & S. pullorum) form black-centre, metallic colonies.
REFERENCES
1. Handbook Culture Media Merck, 1982.
2. Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de microbiologie clinica
ed. III, 2009, 1045-1084.
Disperse culture or pathologic material on the medium surface, then incubate 48 hours
at 37 ºC.
RESULTS
Salmonella strains (excepting S. paratyphi A & S. pullorum) form black-centre, metallic colonies.
REFERENCES
1. Handbook Culture Media Merck, 1982.
2. Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de microbiologie clinica
ed. III, 2009, 1045-1084.
COMPOSITION
Meat extract 5.0 g, peptone from meat 10.0 g, D(+) glucose 5.0 g, Na2HPO4 4.0 g,
iron(II) sulfate 0.3 g, brilliant green 0.025 g, bismuth sulfite indicator 8.0 g, agar-agar
15 g.
Distilled water ad 1000 ml, pH 7.6 ± 0.2, do not autoclave.
Pour in plates. Aspect: grayish yellow to pale green.
Wilson-Blair modified
Beef meat extract 5.0 g, gelatin pancreatic hydrolysate 10.0 g, glucose 5.0 g,
Na2HPO4 4.0 g, iron(II) sulfate 0.3 g, brilliant green 0.025 g, bismuth sulfite indicator
8.0 g, agar 20 g.
Distilled water ad 1000 ml, mix ingredients and heat to boiling max 1-2 min., adjust
pH 7.7, do not autoclave. Cool to 50 ºC and pour in plates.
Meat extract 5.0 g, peptone from meat 10.0 g, D(+) glucose 5.0 g, Na2HPO4 4.0 g,
iron(II) sulfate 0.3 g, brilliant green 0.025 g, bismuth sulfite indicator 8.0 g, agar-agar
15 g.
Distilled water ad 1000 ml, pH 7.6 ± 0.2, do not autoclave.
Pour in plates. Aspect: grayish yellow to pale green.
Wilson-Blair modified
Beef meat extract 5.0 g, gelatin pancreatic hydrolysate 10.0 g, glucose 5.0 g,
Na2HPO4 4.0 g, iron(II) sulfate 0.3 g, brilliant green 0.025 g, bismuth sulfite indicator
8.0 g, agar 20 g.
Distilled water ad 1000 ml, mix ingredients and heat to boiling max 1-2 min., adjust
pH 7.7, do not autoclave. Cool to 50 ºC and pour in plates.
DESCRIPTION
Selective medium for the isolation and differentiation of Salmonella from clinical specimens. Bismuth-sulfite and brilliant green inhibit
the associated microflora. H2S-positive salmonellae form black colonies due to iron sulfide formation. Reduction of bismuth ions to
metallic bismuth produces the metallic look of the colonies.
Selective medium for the isolation and differentiation of Salmonella from clinical specimens. Bismuth-sulfite and brilliant green inhibit
the associated microflora. H2S-positive salmonellae form black colonies due to iron sulfide formation. Reduction of bismuth ions to
metallic bismuth produces the metallic look of the colonies.
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