Thallium acetate may be added to a max concentration of 1/10,000;
addition of pig serum to a final concentration of 20%; pH adjustment to 7.4
Composition of Hank's balanced salt solution (modified)
2 stock solutions are needed:
NaCl 80 g, KCl 4 g, MgSO4 7 H2O 1 g, MgCl2 6 H2O 1 g.
Dissolve in 400 ml water CaCl2 (anhydrous) 1.4 g; add water to 500 ml.
Na2HPO4 12 H2O 1.5 g dissolved in 400 ml water, KH2PO4 0.6 g, Water ad 500 ml.
For use, add 25 ml of stock A to 400 ml of water, followed by 25 ml of stock B; then water ad 500 ml.
Hank's balanced salt solution (modified) 500 ml, Water 750 ml, Bacto brain heart
infusin (Difco) 8.2 g, Bacto PPLO broth w/o CV (Difco) 8.7 g.
Autoclaving at 121 'C for 2-5 minutes.
Yeast extract 60 ml, 0.5% phenol red solution 4.5 ml, Bacitracin 250 mg, Meticillin 250
Medium is used for Mycoplasma hyopneumoniae and Mycoplasma flocculare isolation.
(c) Costin Stoica