Inoculate the culture medium by streaking the plate. Incubate at 37 ºC for 2-7 days.
Blue medium => acetate positive; green medium => acetate negative.
1. Costin I.D.: Utilization of sodium acetate by Shigella and Escherichia. J.Gen. Micrbiol., 41; 23-27, 1965
2. Trabulsi L.R. & Ewing W.H. : Sodium Acetate medium for the differentiation of Shigella and Escherichia cultures. Publ. Hlth. Lab.,
20; 137-140, 1962
3. Handbook Culture Media Merck, 1982.
Sodium chloride 5.0 g, Magnesium sulfate 0.2 g, Ammonium dihydrogen phosphate
1.0 g, Di-potassium hydrogen phosphate 1.0 g, Sodium acetate 2.0 g, Bromthymol
blue 0.08 g, Agar-agar 12.0 g (or 20 g in medium variant).
Distilled water ad 1000 ml, pH 7.0 ± 0.1, sterilize by autoclaving.
Medium for the biochemical differentiation and identification of some Enterobacteriaceae on the basis of acetate degradation.
Degradation of the acetate results in an increase of the pH of the medium, changing colour to blue.
(Trabulsi & Ewing 1962)
(c) Costin Stoica