Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Streptococcaceae, Genus Streptococcus, Streptococcus
pseudopneumoniae Arbique et al. 2005.
Non-groupable by Lancefield antisera.
Gram-positive, spherical to oval cocci, occurring singly or grouped in short chains.
Colonies are smooth, slightly mucoid, grayish, alpha-hemolytic, resembling S.
pneumoniae in appearance. on Trypticase soy agar with 5% sheep blood (SBA)
incubated aerobically for 18 to 24 h at 35 ºC. The appearance of these strains may
vary depending on the base medium, incubation conditions, and age of the colonies.
Facultative anaerobe. Susceptible to optochin when incubated in an ambient
environment by the disk assay, but resistant (no inhibition zone) or indeterminate
(inhibition zone, 8 to 13 mm) when CO2 incubated. Insoluble in bile.
Found in the upper respiratory tract.
May have pathogenic potential.
- Judy C. Arbique, Claire Poyart, Patrick Trieu-Cuot, Gilles Quesne, Maria da Gloria S. Carvalho, Arnold G. Steigerwalt, Roger E.
Morey, Delois Jackson, Ross J. Davidson, and Richard R. Facklam. Accuracy of Phenotypic and Genotypic Testing for
Identification of Streptococcus pneumoniae and Description of Streptococcus pseudopneumoniae sp. nov. J. Clin. Microbiol.,
2004, 42, 4686-4696.
Important for identification are optochin (ethyl hydrocuprein hydrochloride) and bile solubility tests.
Positive results for beta-galactosidase and leucine arylamidase.
Negative results for alkaline phosphatase, arginine dihydrolase, alpha-galactosidase, hydrolysis of hippurate, pyrrolidonyl arylamidase,
Voges-Proskauer reaction, beta-glucuronidase, acid production from: arabinose, glycogen, mannitol, inulin, raffinose, ribose, sorbitol,
starch & trehalose.
Variable results for esculin hydrolysis and acid production from glycerol & lactose.
(c) Costin Stoica