Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Streptococcaceae, Genus Streptococcus, Streptococcus orisasini
Takada et al. 2013.
Member of the "Mutans group".
No reaction with Lancefield antisera (Oxoid kit).
Gram-positive, nonspore-forming cocci, occurring in pairs or short chains.
Colonies on Blood agar are small and white, 0.75–1.0 mm in diameter and non-
haemolytic at 37 ºC for 24 h. Forms small, dark blue, crinkled colonies on MS agar.
Isolated from clinical specimens from donkey oral cavities. Resistant to bacitracin.
Undetermined. Produces glucosyltransferase involved in dental plaque formation (a water-insoluble glucan is produced by this enzyme
- Kazuko Takada, Masanori Saito, Osamu Tsudukibashi, Takachika Hiroi and Masatomo Hirasawa 2013. Streptococcus orisasini
sp. nov. and Streptococcus dentasini sp. nov., isolated from the oral cavity of donkeys. Int. J. Syst. Evol. Microbiol., 63, 2782-2786.
A water-insoluble glucan is produced from sucrose by glucosyltransferase.
Most tests were performed using Rapid ID32 Strep, API 50CH and API ZYM.
Positive results for alanine phenylalanine proline arylamidase, alpha-glucosidase, acid phosphatase, leucine arylamidase, valine
arylamidase, cystine arylamidase and naphthol-AS-BI-phosphohydrolase, Voges-Proskauer reaction, acid production from:
N-acetylglucosamine, amygdalin, D-arabitol, arbutin, aesculin, cellobiose, dulcitose, fructose, glucose, galactose, inulin, lactose,
maltose, D-mannose, D-mannitol, raffinose, D-sorbitol, salicin, sucrose, D-tagatose & trehalose.
Negative for arginine dihydrolase, alkaline phosphatase, catalase, beta-glucuronidase, beta-glucosidase, alpha-galactosidase,
beta-galactosidase, hippurate hydrolysis, N-acetyl-beta-glucosaminidase, acid production from: L-arabinose, glycogen, methyl
beta-D-glucopyranoside, melibiose, melezitose, rhamnose & D-ribose.
(c) Costin Stoica