Staphylococcus saprophyticus
Cultural characteristics
Biochemical characters
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Staphylococcaceae, Genus Staphylococcus,
- Staphylococcus saprophyticus subsp. bovis  Hajek et al. 1996
- Staphylococcus saprophyticus subsp. saprophyticus  (Fairbrother 1940) Shaw et al. 1951
Gram-positive cocci, 0.6-1.4 μm diameter, nonmotile, nonsporing.
Colonies are circular, slightly convex, S-type, pale orange pigmented, 4-9 mm in
diameter. Uniform turbidity in broth cultures followed by clearing with a fine or slightly
mucoid deposit. Facultatively anaerobic, 10-40 ºC, optimum 28-35 ºC. Grow on:
Trypticase Soy Yeast extract Agar ± 5% sheep blood, P agar, Trypticase Soy Yeast
extract  Broth.
Isolated from skin & urine samples (subsp. saprophyticus) and from upper respiratory tract of healthy cows (subsp. bovis).
Present in air, soil, dust, dairy products and surface of animal carcasses. Resistant to novobiocin and lysozyme. Sensible to
Subspecies bovis - unknown (none).
Subspecies saprophyticus - urinary tract infections especially in young women. Virulence factors: adherence to uroepithelial cells,
production of a hemagglutinin, production of extracellular slime.
  1. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Wiliams S.T., 1994. Bergey's Manual of Determinative Bacteriology, Ninth Edition,
    Williams & Wilkins, Baltimore. Group 17, Gram-Positive Cocci, 527-558.
  2. Hajek V., Meugnier H., Bes M., Brun Y., Fiedler F., Chmela Z., Lasne Y., Fleurette J. & Freney J.: Staphylococcus saprophyticus
    subsp. bovis subsp. nov., isolated from bovine nostrils. Int. J. Syst. Bacteriol., 1996, 46, 792-796.
  3. Fairbrother R.W.: Coagulase production as a criterion for the classification of the staphylococci. Journal of Pathology and
    Bacteriology, 1940, 50, 83-88.
  4. Shaw C., Stitt J.M. & Cowan S.T.: Stapyylococci and their classification. Journal of General Microbiology, 1951, 5, 1010-1023.
  5. Karl-Heinz Schleifer and Julia A. Bell, 2009. Family VIII. Staphylococcaceae fam. nov.. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R.
    Krieg, W. Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The
    Firmicutes, Springer, 392-426.
Subsp. bovis
Positive results for benzidine test, catalase, hippurate hydrolysis, nitrate reduction,
urease, acid production from: mannitol, glycerol, N-acetylglucosamine, fructose,
glucose, maltose, ribose & trehalose.

Negative results for arginine dihydrolase, clumping factor, coagulase, elastase, fibrinolysin, heat-labile and heat-stable nucleases,
hyaluronidase, hemolysin, ornithine decarboxylase, esculin hydrolysis, starch hydrolysis, utilization of citrate, H
2S production, acid
production from: adonitol, dulcitol, cellobiose, raffinose, rhamnose & xylose.

Variable results for gelatinase; caseinase; beta-galactosidase, beta-glucuronidase, lecithinase, hydrolysis of Tween 20, Tween 40,
and Tween 80, alkaline phosphatase,  acetoin production, acid production from: arabinose, galactose, lactose, mannose, sucrose &  

Subsp. saprophyticus
Positive results for acetoin production, benzidine test, catalase, urease, acid production from: fructose, glucose, glycerol, maltose,
sucrose, trehalose & turanose.

Negative results for coagulase, DN-ase, esculin hydrolysis, hemolysin, nitrate reduction, ornithine decarboxylase, phosphatase, acid
production from: adonitol, arabinose, arabitol, cellobiose, dulcitol, erythritol, erythrose, fucose, galactose, gentiobiose, inositol, lyxose,
mannose, melibiose, melezitose, raffinose, rhamnose, ribose, salicin, sorbitol, sorbose, tagatose & xylose.
(c) Costin Stoica
Culture media
Biochemical tests
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