Produces tuberculosis in man and animals.
Experimentally, from an inoculum of 0.01 mg,
Mycobacterium tuberculosis is highly pathogenic for guinea pigs and hamsters, but
relatively non-pathogenic for bovines, cats, domestic fowls, goats, and rabbits. Inocula of 0.001-1 mg produce experimental disease
in mice.
Mycobacterium tuberculosis
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium,
Mycobacterium tuberculosis (Zopf 1883) Lehmann and Neumann 1896, type species of the genus.

Old synonyms: "Bacterium tuberculosis" Zopf 1883, "Bacillus tuberculosis" (Zopf 1883) Klein 1884, "Mycobacterium tuberculosis
typus humanus" Lehmann and Neumann 1907, "Mycobacterium tuberculosis var. hominis" Bergey et al. 1934.

Mycobacterium tuberculosis complex comprises Mycobacterium tuberculosis, M. africanum, M. canettii, M. bovis, M. microti, M. orygis,
M. caprae, M. pinnipedii, M. suricattae,  M. mungi, M. dassie,
and M. oryx.
According to Riojas et al. (2018), “phylogenomic analysis of the species of the Mycobacterium tuberculosis complex demonstrates
that
M. africanum, M. bovis, M. caprae, M. microti and M. pinnipedii are later heterotypic synonyms of Mycobacterium tuberculosis”.

"Mycobacterium canetii" does not differ from
Mycobacteriurn tuberculosis in the biochemical tests and in its 16s rRNA sequence, but
produce smooth and glossy colonies.
According to Jakko van Ingen et al. the "oryx bacillus" is a phylogenetically distinct lineage of the clonal
M. tuberculosis complex and
thus deserves a separate subspecies status as
Mycobacterium orygis.
Acid-alcohol-fast, straight or slightly curved rods, 0.3-0.6 / 1-4 μm, occurring singly
and in occasional threads. Generation time in vitro under optimal conditions is 14-15
hours. Most strains can produce cords, a virulence-related character.
Colonies are rough, raised, and thick, with a nodular or wrinkled surface and an
irregular thin margin; may become somewhat pigmented (off-white to faint buff, or
even yellow). Colonies on oleic acid-albumin agar are flat, rough, corded, dry, and
usually non-pigmented. In liquid media, old cultures produce a thick and wrinkled
pellicle. Grows on Lowenstein-Jensen with glycerol or sodium piruvate. No growth in
5% NaCl. No growth on MacConkey agar.
Optimum temperature for growth is 37 ºC (some growth occurs at 30-34 ºC).
Optimum pH is 6.4-7.0. Growth is stimulated by adding  5-10% CO
2, and 0.5% (w/v)
glycerol.
Isolated from cases of tuberculosis in man, other primates, dogs and other animals which have contact with man. Resistant to
thiophene-2-carboxylic acid hydrazide (TCH). Sensitive to rifampin (25 μg/ml), hydroxylamine (500 µg/ml), ethambutol (2 µg/ml) and
isoniazid (1 µg/ml). Variable resistance to streptomycin.
"Mycobacterium canetii" was isolated from  a 2-year-old Somali patient with lymphadenitis.
"Mycobacterium orygis" havs been isolated from members of the Bovidae family, (oryxes, gazelles, deer, antelope, and waterbucks),
although their exact host range remains unsettled.
Infected animals, including man, exhibit delayed hypersensitivity to crude or purified
Mycobacterium tuberculosis culture filtrates (tuberculins) and less sensitivity to
tuberculin-like preparations from other mycobacteria. Disease caused by
Mycobacterium bovis cannot be distinguished from that due to Mycobacterium
tuberculosis
by use of commonly available tuberculins.

Bacillus Calmette-Guérin (BCG) is a live attenuated
Mycobacterium bovis strain used
to prevent tuberculosis and other mycobacterial infections. The vaccine was
developed by Calmette and Guérin and was first administered to human beings in
1921. BCG is the only vaccine against tuberculosis.

"Mycobacterium orygis" is causative agents of tuberculosis in the animal species.
Recently it was reported as a cause of human tuberculosis in patients of South Asian
origin.
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  4. Marco A. Riojas, Katya J. McGough, Cristin J. Rider-Riojas, Nalin Rastogi and Manzour Hernando Hazbón. Phylogenomic analysis
    of the species of the Mycobacterium tuberculosis complex demonstrates that Mycobacterium africanum, Mycobacterium bovis,
    Mycobacterium caprae, Mycobacterium microti and Mycobacterium pinnipedii are later heterotypic synonyms of Mycobacterium
    tuberculosis. Int J Syst Evol Microbiol. 2018 Jan;68(1):324-332. doi: 10.1099/ijsem.0.002507.
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    1348-0421.1985.tb00894.x
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    p. 409-420.
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  13. Turenne C, Chedore P, Wolfe J, Jamieson F, Broukhanski G, May K, Kabani A. Mycobacterium lacus sp. nov., a novel slowly
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  14. Koksalan OK, Aydin MD, Eraslan S, Bekiroglu N. Reliability of cord formation in BACTEC 12B/13A media for presumptive
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    54. doi:10.20506/rst.20.1.1265
Positive results for acid phosphatase, alpha-esterase, niacin production (niacin-negative strains may be found in patients who have
received long-term chemotherapy), nitrate reduction, nicotinamidase, pyrazinamidase, Tween 80 hydrolysis (most strains) and urease.

Negative results for catalase (inactivated at 68 ºC), semiquantitative catalase test, arylsulphatase (3 and 10 days), beta-galactosidase,
iron uptake, and tellurite reduction (variable / positive at 9 days strains).
No utilization as sole carbon source of acetate, citrate, succinate, malate, pyruvate, benzoate, fumarate, glucose, fructose, sucrose
ethanol, and propanol.
(c) Costin Stoica
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