Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium triplex Floyd et al. 1997.
Member of the Mycobacterium simiae complex.
Acid-alcohol-fast, short rods to coccoid forms.
Colonies on Lowenstein–Jensen medium and Middlebrook 7H10 agar after 14-21
days are cream to buff, non-pigmented, usually smooth, sometimes appearing rough
with age. Temperature range for growth is 30-37 ºC; does not grow at 25, 42 or 52 ºC.
Does not grow on MacConkey agar w/o crystal violet or on media supplemented with
5% (w/v) NaCl.
Isolated from isolated from cerebrospinal fluid, lymph nodes, bronchoalveolar lavage and sputum (in USA, Northern Ireland and Italy).
Isolated from the gills of perch, Perca fluviatilis L., captured in a reservoir in the Czech Republic.
Resistant to thiophen-2-carboxylic acid hydrazide (5.0 μg/ml), rifampin (6.0 μg/ml), isoniazid (1 μg/ml), ethambutol (2 μg/ml), and
streptomycin (8 μg/ml).
May cause pulmonary disease in immunocompromised / immunocompetent persons.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Piersimoni C, Zitti P, Mazzarelli G, Mariottini A, Nista D, Zallocco D. Mycobacterium triplex pulmonary disease in
immunocompetent host. Emerg Infect Dis. 2004;10(10):1859–1862. doi:10.3201/eid1010.040217.
- Floyd MM, Guthertz LS, Silcox VA, Duffey PS, Jang Y, Desmond EP, Crawford JT, Butler WR. Characterization of an SAV organism
and proposal of Mycobacterium triplex sp. nov. J Clin Microbiol 1996; 34:2963-2967.
- McMullan R, Xu J, Kelly M, et al. Mycobacterium triplex pulmonary infection in an immunocompetent patient. J Infect. 2002;44(4):
Positive results for semiquantitative catalase test, heat-stable catalase (68 ºC), nitrate reduction, and urea hydrolysis.
Negative results for arylsulphatase (3 days, variable at 14 days), niacin accumulation, tellurite reduction (one isolate) and Tween 80
(c) Costin Stoica