Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium tokaiense (ex Tsukamura 1973) Tsukamura 1981.
Acid-fast long rods, 1-7 μm, sometimes cross-barred. Prolonged culture may lose
some acid-fastness. Mycelium is not formed. No cord formation.
Dilute inocula on egg media yield smooth, scotochromogenic colonies in less than 5
days. Grows at 25-37 ºC, but not at 45 or 52 ºC.
Isolated from human sputum and from soil.
Susceptible to hydroxylamine (500 μg/ml) and ethambutol (5 μg/ml). Resistant to thiophene-2-carboxylic acid hydrazide (1 μg/ml), and
rifampin (25 μg/ml).
Considered to be non-pathogenic.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Tsukamura M, Mizuno S, Tsukamura S. Numerical analysis of rapidly growing, scotochromogenic mycobacteria, including
Mycobacterium obuense sp. nov., nom. rev., Mycobacterium rhodesiae sp. nov., nom. rev., Mycobacterium aichiense sp. nov.,
nom. rev., Mycobacterium chubuense sp. nov., nom. rev., and Mycobacterium tokaiense sp. nov., nom. rev. Int. J. Syst. Bacteriol.
- Ramaprasad EV, Rizvi A, Banerjee S, Sasikala C, Ramana CV. Mycobacterium oryzae sp. nov., a scotochromogenic, rapidly
growing species is able to infect human macrophage cell line. Int J Syst Evol Microbiol 2016; 66:4530-4536.
Positive results for arylsulfatase (3 and 7 days), alpha-esterase, beta-esterase, beta-galactosidase, acetamidase, benzamidase,
isonicotinamidase, alantoinase, succinamidase, nicotinamidase, pyrazinamidase, and urease.
Can utilize as sole carbon source acetamide, acetate, succinate, malate, fumarate, fructose, glucose, pyruvate, ethanol, sucrose,
N-acetyl-Dglucosamine, D-mannose, L-rhamnose, D-xylose, inositol, D-mannitol, D-sorbitol, acetate, DL-lactate, pyruvate, and
Negative results for acid phosphatase, catalase, semiquantitative catalase test, niacin production, nitrate reduction, salicylamidase,
and Tween 80 hydrolysis (positive after 14 days incubation).
No utilization of benzoate, citrate, cellobiose, L-serine and malonate.
(c) Costin Stoica