Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium sphagni Kazda 1980.
Acid-alcohol-fast, short, thick, often pleomorphic rods; usually clumped. No cording.
Gram-positive. No spores, capsules, true branching, or aerial hyphae are produced.
Eugonic, smooth, glistening, orange-yellow scotochromogenic colonies are produced
from dilute inocula on Lowenstein-Jensen and Middlebrook 7H10 agar media. The
temperature range for growth is 22-37 ºC; optimum at 31 ºC; does not grow at 42, 45
or 52 ºC. Does not grow on MacConkey agar (without crystal violet) or in the presence
of 3% (w/v) NaCl.
Isolated from sphagnum vegetation in moors of Germany and Scandinavia (Sphagnum recurvum).
Susceptible to hydroxylamine (500 μg/ml) and ethambutol.
No demonstrable virulence for rabbits, guinea pigs, or mice injected with 1-10 mg wet weight of cells.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Tsukamura M, Mizuno S, Tsukamura S. Numerical analysis of rapidly growing, scotochromogenic mycobacteria, including
Mycobacterium obuense sp. nov., nom. rev., Mycobacterium rhodesiae sp. nov., nom. rev., Mycobacterium aichiense sp. nov.,
nom. rev., Mycobacterium chubuense sp. nov., nom. rev., and Mycobacterium tokaiense sp. nov., nom. rev. Int. J. Syst. Bacteriol.
- Kazda J. Mycobacterium sphagni sp. nov. Int. J. Syst. Bacteriol. 1980; 30:77-81.
Positive results for acid phosphatase, arylsulfatase (14 days), nitrate reduction, Tween 80 hydrolysis, urease, acid production from
D-fructose, D-glucose, myo-inositol, D-mannitol and trehalose.
Can utilize as sole carbon source malonate, oxalate, and propanol.
Negative results for arylsulfatase (3 days), catalase, alpha- and beta-esterase, beta-galactosidase, acetamidase, benzamidase,
isonicotinamidase, nicotinamidase, salicylamidase, allantoinase, succinamidase, malonamidase, iron uptake, niacin test, acid
production from L-arabinose, galactose, L-rhamnose, D-sorbitol, and D-xylose.
No utilization as sole carbon source of benzoate, malate, or hippurate.
Variable results for pyrazinamidase, fumarate, succinate, and citrate utilization.
(c) Costin Stoica