Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium seoulense Mun et al. 2007.
Species description is based on a single strain.
Acid-alcohol-fast, rods; frequently bent and occasionally coccoid. Spores and
filaments are not present.
Micro-colonies may be visible in 2 weeks on Middlebrook 7H10 agar; smooth,
orange-pigmented; mature colonies require incubation for 3 weeks. Growth may be
slower (4 or more weeks) on Löwenstein-Jensen medium and appear as an
orange-colored surface film. Temperature range for growth is 25-37 ºC, optimal
growth is at 37 ºC; does not grow at 45 ºC. Does not grow on media supplemented
with 5% (w/v) NaCl or on MacConkey agar (without crystal violet).
Isolated from human sputum samples in Seoul, Korea.
Resistant to thiophene-2-carboxylic acid hydrazide (TCH), ethambutol (EMB) and picric acid.
Isolated from sputum samples of a 52-year-old female patient experiencing generalized pulmonary symptoms.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Mun HS, Kim HJ, Oh EJ, Kim H, Bai GH, Yu HK, Park YG, Cha CY, Kook YH, Kim BJ. Mycobacterium seoulense sp. nov., a slowly
growing scotochromogenic species. Int J Syst Evol Microbiol 2007; 57:594-599.
Positive results for nitrate reductase, heat-stable catalase (68 ºC), pyrazinamidase and tellurite reduction.
Negative results for arylsulfatase (3 and 14 days), niacin accumulation, Tween 80 hydrolysis, and urease.
(c) Costin Stoica