Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium scrofulaceum Prissick and Masson 1956.
Old synonym: "Mycobacterium marianum".
Acid-fast, short to long rods or filaments. No cord formation.
Colonies on Middlebrook medium and Lowenstein-Jensen medium are usually
smooth and yellow to orange in color, but occasional strains may produce rough
growth. Growth occurs in 7 or more days (28) at 37 ºC. Temperature range for growth
is 22-42 ºC; optimal growth is at 35 ºC. No growth on media supplemented with 5%
(w/v) NaCl, or on MacConkey agar.
First isolated from a closed lesion of cervical lymphadenitis in a child. Also found in human sputum and gastric lavage samples.
Resistant to tiophene-2-carboxylic acid hydrazide (1 µg/ml), hydroxylamine (500 µg/ml), isoniazid (1 µg/ml), and streptomycin (8
µg/ml). Susceptible to ethambutol (2 µg/ml) and rifampin (32 µg/ml).
Rarely found in pus from suppurating cervical lymph nodes, especially in children, and considered to be the etiologic agent of the
lesions. Occasionally associated with pulmonary disease.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Loredana Gabriela Popa, Mircea Ioan Popa 2009. Identificarea bacililor acido-rezistenti in: Tratat de microbiologie clinica, Dumitru
Buiuc, Marian Negut, ed. a III-a, Editura Medicala, 881-890, ISBN (13) 978-973-39-0593-6.
- Tsukamura M. Numerical Classification of Slowly Growing Mycobacteria. International Journal of Systematic Bacteriology, Oct. 1976,
- Stephen Berger 2019. GIDEON Guide to Medically Important Bacteria, eBook.
- Tsukamura M, Yano I, Imaeda T. Mycobacterium fortuitum subspecies acetamidolyticum, a new subspecies of Mycobacterium
fortuitum. Microbiol Immunol 1986; 30:97-110.
- Koksalan OK, Aydin MD, Eraslan S, Bekiroglu N. Reliability of cord formation in BACTEC 12B/13A media for presumptive
identification of Mycobacterium tuberculosis complex in laboratories with a high prevalence of Mycobacterium tuberculosis. Eur J
Clin Microbiol Infect Dis. 2002;21(4):314‐317. doi:10.1007/s10096-002-0701-9.
Positive results for catalase (inactivated at 68°C), semi-quantitative catalase test, and urease.
Can utilize pyruvate, and propionate as sole carbon source in the presence of ammonia.
Negative results for arylsulphatase (3 days), acid phosphatase, beta-galactosidase, iron uptake, niacin production, nitrate reduction,
and Tween 80 hydrolysis.
No utilization of benzoate, citrate, succinate, malate and fumarate.
Variable results for alpha- and beta-esterase, arylsulphatase (10 days), nicotinamidase, pyrazinamidase, and tellurite reduction.
(c) Costin Stoica