Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium rufum Hennessee et al. 2009.
Medium to long, thin rods. Weak Gram-positive. Non-motile.
Colonies within 7 days are large, round, raised, dry with a smooth border and orange
pigmented in light or dark. Grows at 28 ºC (optimally). No growth in media containing
5% NaCl. Aerobe.
Isolated from polycyclic aromatic hydrocarbon-contaminated soil of a former oil-gasification company site in Hilo, Hawaii, USA.
Can degrade/utilize phenanthrene, fluoranthene, but not pyrene.
- Hennessee CT, Seo JS, Alvarez AM, Li QX. Polycyclic aromatic hydrocarbon-degrading species isolated from Hawaiian soils:
Mycobacterium crocinum sp. nov., Mycobacterium pallens sp. nov., Mycobacterium rutilum sp. nov., Mycobacterium rufum sp. nov.
and Mycobacterium aromaticivorans sp. nov. Int J Syst Evol Microbiol 2009; 59:378-387.
Positive results for catalase, alpha-glucosidase, esculin hydrolysis, nitrate reduction and urease.
Can utilize glucose, D-fructose D-mannitol, D-sorbitol, trehalose, D-xylitol, and D-xylose.
Negative results for alkaline phosphatase, beta-glucuronidase, beta-galactosidase, N-acetyl-beta-glucosaminidase, gelatin
hydrolysis, and pyrrolidonyl arylamidase.
No utilization of citrate.
(c) Costin Stoica