Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium pulveris Tsukamura et al. 1983.
Acid-fast short rods or coccoid forms, 0.5 x 2 μm. Mycelium or branching are not
Colonies are wet, smooth, creamy or slightly yellowish, and non-photochromogenic.
Abundant growth appears in 5 d at 37 ºC from dilute inocula on Ogawa egg medium.
The temperature range for growth is 28-42 ºC; occasional strains will grow at 45 ºC.
Grows in the presence of 5% (w/v) NaCl.
Isolated from house dust.
Resistant to ethambutol (5 µg/ml) (most strains), rifampin (25 µg/ml), isoniazid (10 µg/ml), sodium chloride (5%), p-nitrobenzoic acid
(0.5 mg/ml), sodium salicylate (0.5 mg/ml), and thiophene-2-carboxylic acid hydrazide (1 µg/ml). Susceptible to hydroxylamine (500
µg/ml) and 0.2% picric acid.
- Tsukamura M, Mizuno S, Toyama H. Mycobacterium pulveris sp. nov., a nonphotochromogenic mycobacterium with an
intermediate growth rate. Int. J. Syst. Bacteriol. 1983; 33:811-815.
- Tsukamura M, Yano I, Imaeda T. Mycobacterium moriokaense sp. nov., a rapidly growing, nonphotochromogenic Mycobacterium.
Int. J. Syst. Bacteriol. 1986; 36:333-338.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
Positive results for acid phosphatase, arylsulfatase after 14 days, thermostable catalase (68 ºC), alpha- and beta-esterase, nitrate
reduction, nicotinamidase, pyrazinamidase, and Tween 80 hydrolysis.
Can utilize as sole carbon source in the presence of ammoniacal nitrogen: glucose and glutamate.
Negative results for arylsulfatase after 3 days, acetamidase, allantoinase, succinamidase, benzamidase, isonicotinamidase,
semiquantitative catalase activity (<45 mm foam), and niacin production.
No utilization as sole carbon source in the presence of ammoniacal nitrogen of fructose, sucrose, mannose, galactose, arabinose,
rhamnose, inositol, ethanol, propylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, 2,3-butylene glycol, n-butanol, isobutanol, D-
xylose, trehalose, mannitol, sorbitol, citrate, succinate, malate, malonate, benzoate, and fumarate.
Variable results for beta-galactosidase and urease.
(c) Costin Stoica