Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium psychrotolerans Trujillo et al. 2004.
Acid-fast rods. Gram-positive. Non-motile. Non-spore-forming.
Colonies on GYEA, Bennett’s agar and nutrient agar are smooth, entire, bright
orange, scotochromogenic, and appear after 2 days incubation. Growth on
Lowenstein–Jensen agar is moderate. Grows at 4-37 ºC; no growth was recorded at
45 or 52 ºC. Growth occurs in the presence of 7.0% NaCl. No growth on MacConkey
Isolated from pond water near a uranium mine in Salamanca, Spain.
Susceptible to ciprofloxacin, gentamicin, neomycin and oxytetracycline. Resistant to ampicillin, cefuroxime, cloxacillin,
erythromycin, penicillin and polymyxin.
- Trujillo ME, Velazquez E, Kroppenstedt RM, Schumann P, Rivas R, Mateos PF, Martinez-Molina E. Mycobacterium psychrotolerans
sp. nov., isolated from pond water near a uranium mine. Int J Syst Evol Microbiol 2004; 54:1459-1463.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Nouioui I, Brunet LR, Simpson D, Klenk HP, Goodfellow M. Description of a novel species of fast growing mycobacterium:
Mycobacterium kyogaense sp. nov., a scotochromogenic strain received as Mycobacterium vaccae. Int J Syst Evol Microbiol 2018;
Positive results for arylsulphatase (3 and 14 days), acid phosphatase, arginine dehydrolase, allantoinaminidase, catalase, esterase
(2-naphthyl-butyrate), alpha- and beta-glucosidase, lipase (2-naphthyl-caprylate), 2-naphthylmyristate, cysteine arylamidase, leucine
arylamidase, nitrate reduction, pyrazinamidase, phosphohydrolase, tellurite reduction, trypsin, Tween 80 hydrolysis, urease (weak
reaction) / (negative in Nouioui's type strain study), and valine arylamidase.
Can utilize as sole carbon source adonitol, aesculin, erythritol, D-fructose, gluconate, D-glucose, N-acetylglucosamine, glycerol,
inositol, malate, mannitol, D-mannose, sucrose, sorbitol, trehalose and D-xylose.
Negative results for thermostablecatalase (68 ºC), alpha-chymotrypsin, alpha-fucosidase, alpha- and beta-galactosidase,
beta-glucuronidase, N-acetyl-beta-glucosaminidase and alpha-mannosidase.
No utilization of amygdalin, D- and L-arabinose, arbutin, cellobiose, citrate, dulcitol, D- and L-fucose, galactose, beta-gentiobiose,
glycogen, inulin, lactose, maltose, melezitose, melibiose, D-raffinose, rhamnose, ribose, salicin, L-sorbose, starch, D-tagatose,
D-turanose, L-xylose or methyl beta-xyloside.
(c) Costin Stoica