Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium poriferae Padgitt and Moshier 1987.
Strongly acid-fast rods, 0.7-2.5 μm x 1.1-4.9 μm, which frequently appear coccoid.
Gram-positive. No branching or Y-shaped cells occur, but cells may become
enlarged on one end and display an ovoid, non-acid-fast body.
Colonies are smooth, moist, shiny, and dome shaped with a near-translucent apron
which disappears on continued growth. Strains are scotochromogenic producing
a strongly orange coloration. Visible growth appears on various media in 4 days,
including Lowenstein-Jensen medium and Middlebrook 7H11 agar. Tolerate a pH
range of 5.0-8.5. Temperature range for growth is 20-37 ºC; optimal growth occurs at
28-30 ºC. No growth at 42 ºC. Growth occurs in the presence of 5.0% NaCl, but not on
MacConkey agar without crystal violet
Isolated from cell suspensions of a marine sponge and human sputum. Susceptible to hydroxylamine (500 μg/ml). Resistant to
salicylate, picric acid, PAS, and TCH.
- Padgitt PJ, Moshier SE. Mycobacterium poriferae sp. nov., a scotochromogenic, rapidly growing species isolated from a marine
sponge. Int. J. Syst. Bacteriol. 1987; 37:186-191.
- Ballester F, Pujol I, Alcaide F, et al. First human isolate of Mycobacterium poriferae in the sputum of a patient with chronic
bronchitis. J Clin Microbiol. 2011;49(8):3107-3108. doi:10.1128/JCM.00436-11.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Shojaei H, Daley C, Gitti Z, Hashemi A, Heidarieh P, Moore ER, Naser AD, Russo C, van Ingen J, Tortoli E. Mycobacterium
iranicum sp. nov., a rapidly growing scotochromogenic species isolated from clinical specimens on three different continents. Int
J Syst Evol Microbiol 2013; 63:1383-1389.
Positive results for catalase, semiquantitative catalase test, heat-stable catalase (68 ºC), iron uptake, nicotinamidase,
pyrazinamidase, Tween hydrolysis (7 days), urease, acid production from glucose, mannitol, sucrose, D-xylose, and mannose.
Can utilize acetate, pyruvate, citrate, succinate, malate, benzoate, malonate, fumarate, glucose, galactose, trehalose, mannose,
D-xylose, mannitol, sorbitol, fructose, sucrose, ethanol, 1-propanol, propylene glycol, 1-butanol, 2,3-butylene glycol, and glycerol.
Negative results for arylsulfatase (3 days), acetamidase, allantoinase, benzamidase, isonicotinamidase, nitrate reductase,
succinamidase, niacin accumulation, acid production from trehalose, inositol, L-arabinose, galactose, lactose, L-rhamnose or
No utilization of 1,3-butylene glycol, 1,4-butylene glycol, isobutanol, inositol, L-arabinose, or L-rhamnose.
(c) Costin Stoica