Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium phocaicum Adekambi et al. 2006.
Acid-fast bacilli. Gram-positive.
Colonies are non-pigmented and appear on 5% sheep blood agar, Middlebrook
7H10 agar and egg-based Lowenstein-Jensen slants in 2-5 days at temperatures
between 24 and 37 ºC, optimally at 30 ºC. No growth occurs at 42 ºC. No growth in 5%
(w/v) NaCl media or on MacConkey agar without crystal violet.
Associated with chronic pneumonia.
Isolated from human bronchial aspirate, two other strains have been isolated from patients with chronic pneumonia.
Susceptible in vitro to cefoxitin, imipenem, minocycline, doxycycline, clarithromycin, erythromycin, azithromycin, amikacin, ciprofloxacin,
ofloxacin and sparfloxacin. Resistant to amoxycillin, amoxycillin/clavulanate and tobramycin.
- Adekambi T, Berger P, Raoult D, Drancourt M. rpoB gene sequence-based characterization of emerging non-tuberculous
mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium
aubagnense sp. nov. Int J Syst Evol Microbiol 2006; 56:133-143.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Gomila M, Ramirez A, Gasco J, Lalucat J. Mycobacterium llatzerense sp. nov., a facultatively autotrophic, hydrogen-oxidizing
bacterium isolated from haemodialysis water. Int J Syst Evol Microbiol 2008; 58:2769-2773.
Positive results for arylsulfatase (3 days), acetoin production, alkaline phosphatase, esculin hydrolysis, nitrate reductase, and
Can utilize citrate as a sole carbon source.
Negative results for arginine dihydrolase, acid phosphatase, semiquantitative catalase, thermostable catalase, beta-galactosidase,
alpha-glucosidase, beta-glucuronidase, gelatinase, N-acetyl-beta-glucosaminidase, H2S production, indole production, iron uptake,
lysine decarboxylase, ornithine decarboxylase, pyrrolidonyl arylamidase, tryptophan desaminase, Tween 80 hydrolysis, and urease.
(c) Costin Stoica