Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium parascrofulaceum Turenne et al. 2004.
Member of the Mycobacterium simiae complex.
Acid-fast pleomorphic bacilli; moderate beading.
Colonies on Lowenstein–Jensen and Middlebrook 7H10 agar grow within 2-4 weeks
and are scotochromogenic with yellow to orange pigmentation. Most strains form
smooth and domed colonies, but dry-wrinkled and mucoid forms can occur.
Temperature range for growth is 25-37 ºC; optimal growth is at 37 ºC; does not grow
at 42 ºC, on MacConkey agar or on media supplemented with 5% (w/v) NaCl.
Isolated from human respiratory samples. Susceptible to rifampin (32 µg/ml), clarithromycin, amikacin and sulfamethoxazole.
Resistant to isoniazid (1 µg/ml), ethambutol (2 µg/ml) (variable), streptomycin (8 µg/ml) (variabile).
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Turenne CY, Cook VJ, Burdz TV, Pauls RJ, Thibert L, Wolfe JN, Kabani A. Mycobacterium parascrofulaceum sp. nov., novel slowly
growing, scotochromogenic clinical isolates related to Mycobacterium simiae. Int J Syst Evol Microbiol 2004; 54:1543-1551.
Positive results for thermotolerant catalase (68 ºC), pyrazinamidase, and urease.
Negative results for acid phosphatase, arylsulfatase (3 days), alpha- and beta-galactosidase, iron uptake, niacin accumulation,
nitrate reduction, Tween 80 hydrolysis, and tellurite reduction.
Variable results for arylsulfatase (14 days), and semi-quantitative catalase.
(c) Costin Stoica