Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium paraffinicum (ex Davis et al. 1956) Toney et al. 2010.
Acid-alcohol-fast, long, slender rods, 0.5-0.7 x 3.0-7.0 µm. Gram-positive. Non-motile.
Colonies are yellow, waxy and smooth or wrinkled on Lowenstein-Jensen medium.
Grows on Middlebrook 7H10 and 7H11 agar and in 7H9 broth. Growth occurs slowly,
in more than 7 days. Reduced growth at 22 ºC; optimum growth at 35 ºC and no
growth at 42 or 45 ºC. No growth in 5% NaCl.
Isolated below the level of grass roots in oily pools. Abundant in soil samples of petroliferous emanation, principally oilfields. Can
utilize gaseous and liquid paraffinic hydrocarbons.
Susceptible to amikacin, rifabutin, rifampicin, streptomycin, clarithromycin and linezolid. Resistant to ethambutol, ciprofloxacin,
- Toney N, Adekambi T, Toney S, Yakrus M, Butler WR. Revival and emended description of 'Mycobacterium paraffinicum' Davis,
Chase and Raymond 1956 as Mycobacterium paraffinicum sp. nov., nom. rev. Int J Syst Evol Microbiol 2010; 60:2307-2313.
Positive results for semi-quantitative catalase test (>45 mm), heat-stable (68 ºC), and Tween 80 opacity (5 weeks).
Negative results for acid phosphatase, arylsulfatase ( variable after 10 days), nitrate reduction, nicotinamidase, pyrazinamidase,
Tween 80 hydrolysis, and urease.
(c) Costin Stoica