| Mycobacterium novocastrense |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium novocastrense Shojaei et al. 1997.
Mycobacterium, Mycobacterium novocastrense Shojaei et al. 1997.
Weakly acid-alcohol-fast rods, 3-4 µm long; filamentous forms in older cultures.
Gram-positive. Non-motile. Non-spore-forming.
Gram-positive. Non-motile. Non-spore-forming.
Colonies on Lowenstein-Jensen medium and Middlebrook 7H10 agar are
moderately photochromogenic, yellow-pigmented when incubated for 3-7 days in the
light at 30-42 ºC. Older cultures show a deeper yellow pigmentation. Good growth
occurs on Columbia blood agar (3 days); weak growth is formed on MacConkey agar
(without crystal violet) and 5% (w/v) NaCl agar after 14 days.
moderately photochromogenic, yellow-pigmented when incubated for 3-7 days in the
light at 30-42 ºC. Older cultures show a deeper yellow pigmentation. Good growth
occurs on Columbia blood agar (3 days); weak growth is formed on MacConkey agar
(without crystal violet) and 5% (w/v) NaCl agar after 14 days.
Undetermined. May be involved in pulmonary and skin infections.
Isolated from a slowly spreading skin granulation of the hand of a child, tissue biopsy specimen of an apparently healthy adult,
bronchoalveolar lavage, and water (surface and hospital).
Susceptible to capreomycin sulfate (35.5 µg/ml), ciprofloxacin (2.5 µg/ml), cycloserine (16 µg/ml), ethambutol (3.2 µg/ml),
ethionamide (18 µg/ml), and streptomycin sulfate (10 µg/ml). Resistant to hydroxylamine (500 µg/ml), pyrazinamide (66 µg/ml),
rifampin (32 µg/ml), thiacetazone (10 µg/ml), or TCH (thiophen-2-carboxylic acid hydrazide) (5 µg/ml).
bronchoalveolar lavage, and water (surface and hospital).
Susceptible to capreomycin sulfate (35.5 µg/ml), ciprofloxacin (2.5 µg/ml), cycloserine (16 µg/ml), ethambutol (3.2 µg/ml),
ethionamide (18 µg/ml), and streptomycin sulfate (10 µg/ml). Resistant to hydroxylamine (500 µg/ml), pyrazinamide (66 µg/ml),
rifampin (32 µg/ml), thiacetazone (10 µg/ml), or TCH (thiophen-2-carboxylic acid hydrazide) (5 µg/ml).
- Shojaei H, Goodfellow M, Magee JG, Freeman R, Gould FK, Brignall CG. Mycobacterium novocastrense sp. nov., a rapidly growing
photochromogenic mycobacterium. Int J Syst Bacteriol 1997; 47:1205-1207. - Shojaei H, Hashemi A, Heidarieh P, Naser AD. Mycobacterium novocastrense-associated pulmonary and wound infections.
Emerg Infect Dis. 2011;17(3):550-551. doi:10.3201/eid1703.101400. - Varghese B, Enani M, Shoukri M, AlThawadi S, AlJohani S, Al- Hajoj S (2017) Emergence of Rare Species of Nontuberculous
Mycobacteria as Potential Pathogens in Saudi Arabian Clinical Setting. PLoS Negl Trop Dis 11(1): e0005288. - Dibaj R, Azadi D, Karami M, Naser AD, Shojaei H. First report of isolation of Mycobacterium novocastrense from water supplies.
APMIS. 2014 May;122(5):459-61. doi: 10.1111/apm.12165. - John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375. - Balcazar JL, Planas M, Pintado J. Mycobacterium hippocampi sp. nov., a rapidly growing scotochromogenic species isolated from
a seahorse with tail rot. Curr Microbiol 2014; 69:329-333. - Nouioui I, Sangal V, Carro L, Teramoto K, Jando M, Montero-Calasanz MDC, Igual JM, Sutcliffe I, Goodfellow M, Klenk HP. Two
novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov. Int J
Syst Evol Microbiol 2017; 67:4948-4955.
Positive results for esterase (C4), alkaline phosphatase, arylsulfatase (14 days), catalase (45 mm foam), and nitrate reductase.
Can utilize citric acid.
Negative results for arylsulfatase (3 days), heat-stable catalase (68 ºC), beta-galactosidase, alpha- and beta-glucosidase, iron
uptake, niacin production, tellurite reduction, and Tween 80 hydrolysis. No utilization of D- and L- malic acid.
Contradictory results for urease activity.
Can utilize citric acid.
Negative results for arylsulfatase (3 days), heat-stable catalase (68 ºC), beta-galactosidase, alpha- and beta-glucosidase, iron
uptake, niacin production, tellurite reduction, and Tween 80 hydrolysis. No utilization of D- and L- malic acid.
Contradictory results for urease activity.
(c) Costin Stoica
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