Mycobacterium novocastrense
Cultural characteristics
Biochemical characters
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium novocastrense Shojaei et al. 1997.
Weakly acid-alcohol-fast rods, 3-4 µm long;  filamentous forms in older cultures.
Gram-positive. Non-motile. Non-spore-forming.
Colonies on Lowenstein-Jensen medium and Middlebrook 7H10 agar are
moderately photochromogenic, yellow-pigmented when incubated for 3-7 days in the
light at 30-42 ºC. Older cultures show a deeper yellow pigmentation. Good growth
occurs on Columbia blood agar (3 days); weak growth is formed on MacConkey agar
(without crystal violet) and 5% (w/v) NaCl agar after 14 days.
Undetermined. May be involved in pulmonary and skin infections.
Isolated from  a slowly spreading skin granulation of the hand of a child, tissue biopsy specimen of an apparently healthy adult,  
bronchoalveolar lavage, and water (surface and hospital).
Susceptible to capreomycin sulfate (35.5 µg/ml), ciprofloxacin (2.5 µg/ml), cycloserine (16 µg/ml), ethambutol (3.2 µg/ml),
ethionamide (18 µg/ml), and streptomycin sulfate (10 µg/ml). Resistant to hydroxylamine (500 µg/ml), pyrazinamide (66 µg/ml),
rifampin (32 µg/ml), thiacetazone (10 µg/ml), or TCH (thiophen-2-carboxylic acid hydrazide) (5 µg/ml).
  1. Shojaei H, Goodfellow M, Magee JG, Freeman R, Gould FK, Brignall CG. Mycobacterium novocastrense sp. nov., a rapidly growing
    photochromogenic mycobacterium. Int J Syst Bacteriol 1997; 47:1205-1207.
  2. Shojaei H, Hashemi A, Heidarieh P, Naser AD. Mycobacterium novocastrense-associated pulmonary and wound infections.
    Emerg Infect Dis. 2011;17(3):550-551. doi:10.3201/eid1703.101400.
  3. Varghese B, Enani M, Shoukri M, AlThawadi S, AlJohani S, Al- Hajoj S (2017) Emergence of Rare Species of Nontuberculous
    Mycobacteria as Potential Pathogens in Saudi Arabian Clinical Setting. PLoS Negl Trop Dis 11(1): e0005288.
  4. Dibaj R, Azadi D, Karami M, Naser AD, Shojaei H. First report of isolation of Mycobacterium novocastrense from water supplies.
    APMIS. 2014 May;122(5):459-61. doi: 10.1111/apm.12165.
  5. John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
    Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
  6. Balcazar JL, Planas M, Pintado J. Mycobacterium hippocampi sp. nov., a rapidly growing scotochromogenic species isolated from
    a seahorse with tail rot. Curr Microbiol 2014; 69:329-333.
  7. Nouioui I, Sangal V, Carro L, Teramoto K, Jando M, Montero-Calasanz MDC, Igual JM, Sutcliffe I, Goodfellow M, Klenk HP. Two
    novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov. Int J
    Syst Evol Microbiol 2017; 67:4948-4955.
Positive results for esterase (C4), alkaline phosphatase, arylsulfatase (14 days), catalase (45 mm foam), and nitrate reductase.
Can utilize citric acid.

Negative results for arylsulfatase (3 days),  heat-stable catalase (68 ºC), beta-galactosidase, alpha- and beta-glucosidase, iron
uptake, niacin production, tellurite reduction, and Tween 80 hydrolysis. No utilization of D- and L- malic acid.

Contradictory results for urease activity.
(c) Costin Stoica
Culture media
Biochemical tests
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