Mycobacterium nonchromogenicum
Cultural characteristics
Biochemical characters
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium nonchromogenicum Tsukamura 1965.

Member of the
Mycobacterium terrae complex.
Acid-alcohol-fast, moderately long to long rods. Gram-stain-positive.
Colonies on Lowenstein–Jensen medium and Middlebrook agar are smooth to rough
and white to buff after incubation for 7 or more days at 37 ºC. Grows at 22-37 ºC. Weak
growth at 42 ºC and no growth  at 45 ºC. Growth time varies between 12 and 28 days.
No growth on 5% (w/v) NaCl.
Isolated from soil, water and bovine nasal swabs. Environmental contaminant of clinical specimens. One isolate from roach skin
Rutilus rutilus).
Susceptible to ethambutol (2 µg/ml). Resistant to TCH (1 µg/ml), hydroxylamine (500 µg/ml), isoniazid (1 µg/ml), rifampin (25 μg/ml),
and streptomycin (2 µg/ml).
Isolated from mice injected with soil. Bacilli persist in tissues of mice without evidence of pathogenicity.
  1. John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
    Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
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    Buiuc, Marian Negut, ed. a III-a, Editura Medicala, 881-890, ISBN (13) 978-973-39-0593-6.
  3. George P. Kubica, Vella A. Silcox, James O. Kilburn, Ronald W. Smithwick, R. Edward Beam, Wilbur D. Jones, Jr. , and K. D.
    Stottmeier. Differential Identification of Mycobacteria VI. Mycobacterium triviale Kubica sp. nov. International Journal of Systematic
    Bacteriology Vol. 20, No. 2 April 1970 PP. 161-174.
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    Mycobacterium Triviale. American Journal of Respiratory and Critical Care Medicine 2016;193:A3752.
  5. Tsukamura M. Numerical identification of slowly growing mycobacteria. Microbiol Immunol. 1985;29(11):1039‐1050. doi:10.1111/j.
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    1976, p. 409-420.
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  8. Mrlik V, Slany M, Kubecka J, Seda J, Necas A, Babak V, Slana I, Kriz P, Pavlik I. 2012. A low prevalence of mycobacteria in
    freshwater fish from water reservoirs, ponds and farms. J. Fish Dis. 35:497–504. doi:10.1111/j.1365-2761.2012.01369.x
  9. Lee H, Lee SA, Lee IK, Yu HK, Park YG, Jeong J, Lee SH, Kim SR, Hyun JW, Kim K, et al. Mycobacterium paraterrae sp. nov.
    recovered from a clinical specimen: novel chromogenic slow growing mycobacteria related to Mycobacterium terrae complex.
    Microbiol Immunol 2010; 54:46-53.
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    54. doi:10.20506/rst.20.1.1265.
  11. Gcebe N, Rutten V, Gey van Pittius NC, Michel A. Prevalence and distribution of non-tuberculous mycobacteria (NTM) in cattle,
    African buffaloes (Syncerus caffer) and their environments in South Africa. Transbound Emerg Dis 2013;60:74-84.
Positive results for acid phosphatase, arylsulfatase (10 days, negative in 3 days), catalase (inactivated at 68 ºC), catalase
(semi-quantitative), beta-galactosidase, nicotinamidase, pyrazinamidase, and Tween 80 hydrolysis.
Can utilize as sole carbon source acetate and pyruvate.

Negative results for alpha-esterase, iron uptake, niacin production, nitrate reduction, tellurite reduction (9 days), and urea hydrolysis.
No utilization as sole carbon source of citrate, malate, benzoate, fumarate, glucose, fructose, sucrose ethanol, and propanol.

Variable results for succinate utilization in the presence of ammoniac nitrogen.
(c) Costin Stoica
Culture media
Biochemical tests
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