Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium neglectum Nouioui et al. 2019.
Acid-alcohol-fast rods. Gram-positive.
Colonies after growth on Middelbrook 7H10, proteose peptone-meat extract-glycerol
and Lowenstein-Jensen media following incubation for 5 days at 28 ºC are
nonchromogenic. Grows between 25 and 28 ºC, optimally at 28 ºC and at pH 7.
moderate growth was observed at 20, 25 and 37 ºC. No growth at 4, 15, 42, or 45 ºC.
Strict aerobe. Grows in the presence of potassium tellurite. No growth in 5% NaCl.
Isolated from a biofilm of an underground drinking water system in Germany.
- Nouioui I, Carro L, Sangal V, Jando M, Igual JM, Goodfellow M, Klenk HP. Formal description of Mycobacterium neglectum sp. nov.
and Mycobacterium palauense sp. nov., rapidly growing actinobacteria. Antonie Van Leeuwenhoek 2018; 111:1209-1223.
Positive results for arylsulfatase (3 and 14 days), catalase, thermostable catalase (68 ºC), niacin, nitrate reductase, Tween 80
hydrolysis, and urease.
Can utilize D- and L-malic acid.
No utilization of citric acid. Nouioui's paper contains more utilization substrates in GEN III Biolog system.
(c) Costin Stoica