Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium nebraskense Mohamed et al. 2004.
Acid-fast rods. Non-spore-forming.
Colonies are rough with an elevated centre and produce strong yellow pigmentation
in the dark. Growth time is about 3 weeks on Middlebrook 7H11 medium and 4 or
more weeks on Lowenstein-Jensen medium at 25-35 ºC, with optimal growth at
30-35 ºC and no growth at 42 ºC. No growth on MacConkey agar without crystal violet,
or on media with 5 % NaCl.
Isolated from human sputum (5 strains) in Nebraska, from cavitary lung lesion (1 strain) in New York, and from one patient with
underlying emphysema who presented with cough and hemoptysis.
May produce lung disease.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Mohamed AM, Iwen PC, Tarantolo S, Hinrichs SH. Mycobacterium nebraskense sp. nov., a novel slowly growing
scotochromogenic species. Int J Syst Evol Microbiol 2004; 54:2057-2060.
- Omar Abdulfattah, Antony Lixon, Saroj Kandel, Ebad Ur Rahman, Sasmit Roy, Sumit Dahal, Zainab Alnafoosi, and Frances
Schmidt . Rare case of Mycobacterium nebraskense presenting as asymptomatic cavitary lung lesion. J Community Hosp Intern
Med Perspect. 2018; 8(1): 32–34.
- Mun HS, Kim HJ, Oh EJ, Kim H, Bai GH, Yu HK, Park YG, Cha CY, Kook YH, Kim BJ. Mycobacterium seoulense sp. nov., a slowly
growing scotochromogenic species. Int J Syst Evol Microbiol 2007; 57:594-599.
Positive results for heat-stable catalase (68°C).
Negative results for semi-quantitative catalase test, nitrate reductase, urease activity, niacin accumulation, arylsulfatase activity at 3 and
14 days, and pyrazinamidase production.
Variable results for tellurite reduction and Tween 80 hydrolysis.
(c) Costin Stoica