Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium montefiorense Levi et al. 2003.
Member of the Mycobacterium simiae complex.
Beaded acid-fast rods. Cocco-bacillary forms occur after long incubation (20 weeks)
on sheep blood agar. Resists Gram staining.
Colonies on Middlebrook agar are small, transparent, non-chromogenic, and
biochemically inactive. Colonies on sheep blood agar are rough and alpha-hemolytic.
Growth occurs at 22-25 ºC, but not at 30 ºC or above. Does not grow on media
supplemented with 5% (w/v) NaCl.
Isolated from a granulomatous skin lesion of a moray eel.
Considered to be the etiological agent of granulomatous skin disease in moray eels.
Experimental infection: scratch inoculation of mycobacterium produces lesions in recipient eels.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Levi MH, Bartell J, Gandolfo L, Smole SC, Costa SF, Weiss LM, Johnson LK, Osterhout G, Herbst LH. Characterization of
Mycobacterium montefiorense sp. nov., a novel pathogenic Mycobacterium from moray eels that is related to Mycobacterium
triplex. J Clin Microbiol 2003; 41:2147-2152.
Positive results for heat-stable catalase (68 ºC) (weak reaction).
Negative results for arylsulfatase (3 and 10 days), semiquantitative catalase test, niacin accumulation, nitrate reduction, Tween 80
hydrolysis, and urease.
(c) Costin Stoica