Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium minnesotense Hannigan et al. 2013.
Member of the Mycobacterium terrae complex.
Acid-fast, curved or straight rods. Gram-positive. Nonmotile. Nonspore-forming.
Colonies on Middlebrook 7H11 agar enriched with OADC are smooth, pinkish-orange,
photochromogenic after 7-10 days. Grows at 28-34 ºC (optimum at 30 ºC). No growth
at 37 ºC or on 7H11 plates without OADC. No growth on MacConkey agar without
crystal violet. Grows on media supplemented with 5% (w/v) NaCl.
Isolated from a sphagnum peat bog in northern Minnesota, USA.
Susceptible to neomycin, ampicillin and ciprofloxacin. Resistant to gentamicin, clindamycin, erythromycin, tetracycline, bacitracin,
chloramphenicol, streptomycin, penicillin and sulfisoxazol.
- Hannigan GD, Krivogorsky B, Fordice D, Welch JB, Dahl JL. Mycobacterium minnesotense sp. nov., a photochromogenic
bacterium isolated from sphagnum peat bogs. Int J Syst Evol Microbiol 2013; 63:124-128.
Positive results for arylsulfatase (14 days), heat-stable catalase (68 ºC), and Tween 80 hydrolysis.
Negative results for arylsulfatase (3 days), nitrate reduction, and urease.
(c) Costin Stoica