| Mycobacterium madagascariense |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium madagascariense Kazda et al. 1992.
Mycobacterium, Mycobacterium madagascariense Kazda et al. 1992.
Acid-alcohol-fast, polymorphic rods (0.6 x 1.2-1.5 μm) which often form clumps but not
cords or cross bands. No production of spores, capsules, and aerial hyphae. Gram-
positive.
cords or cross bands. No production of spores, capsules, and aerial hyphae. Gram-
positive.
Colonies on Lawenstein-Jensen medium and on Middlebrook 7H10 agar are smooth,
glistening with yellow or orange pigmentation, and 1 to 2 mm in diameter. The optimum
growth temperature is 31 ºC; no growth occurs at 37, 42 or 45 ºC. At 31 ºC growth was
observed after 3 days; at 22 ºC growth was observed after 7 days. No growth on
MacConkey agar or in 3% (w/v) NaCl media.
glistening with yellow or orange pigmentation, and 1 to 2 mm in diameter. The optimum
growth temperature is 31 ºC; no growth occurs at 37, 42 or 45 ºC. At 31 ºC growth was
observed after 3 days; at 22 ºC growth was observed after 7 days. No growth on
MacConkey agar or in 3% (w/v) NaCl media.
Experimental infection in rabbits, guinea pigs or mice produced neither organic nor local lesions.
Isolated from sphagnum vegetation in Madagascar. One isolate from the sputum of a patient with tracheobronchitis.
Susceptible to hydroxylamine (250 μg/ml), NaNO4 (0.1%). Resistant to sodium azide (20 μg/ml) (3 of 4 strains).
Susceptible to hydroxylamine (250 μg/ml), NaNO4 (0.1%). Resistant to sodium azide (20 μg/ml) (3 of 4 strains).
- Kazda J, Müller HJ, Stackebrandt E, Daffe M, Müller K, Pitulle C. Mycobacterium madagascariense sp. nov. Int. J. Syst. Bacteriol.
1992; 42:524-528. - John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375. - Ballester F, Alcaide F, Pujol I, Hernández-Flix S, Simó JM, Joven J, Camps J. First human isolate of Mycobacterium
madagascariense in the sputum of a patient with tracheobronchitis. Clin Chem Lab Med. 2013 Feb;51(2):e35-6. doi: 10.1515
/cclm-2012-0321.
Positive results for acid phosphatase, arylsulfatase (3 days), catalase, iron uptake, pyrazinamidase, Tween hydrolysis (10 days), and
urease, acid production from: D-glucose, L-arabinose, dulcitol, D-fructose, D-mannitol, L-rhamnose, D-sorbitol (3 of 4 strains),
trehalose and D-xylose
Negative results for acetamidase, benzamidase, isonicotinamidase, malonamidase, nitrate reductase, salicylamidase, and
succinamidase,
No utilization as sole carbon source of sodium benzoate, sodium fumarate, sodium succinate, sodium citrate, sodium malonate,
sodium oxalate, sodium hippurate and 1-propanol.
Variable results for alantoinase, nicotinamidase, putrescine oxidase (24 h), acid production from: D-galactose and inositol.
urease, acid production from: D-glucose, L-arabinose, dulcitol, D-fructose, D-mannitol, L-rhamnose, D-sorbitol (3 of 4 strains),
trehalose and D-xylose
Negative results for acetamidase, benzamidase, isonicotinamidase, malonamidase, nitrate reductase, salicylamidase, and
succinamidase,
No utilization as sole carbon source of sodium benzoate, sodium fumarate, sodium succinate, sodium citrate, sodium malonate,
sodium oxalate, sodium hippurate and 1-propanol.
Variable results for alantoinase, nicotinamidase, putrescine oxidase (24 h), acid production from: D-galactose and inositol.
(c) Costin Stoica
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