| Mycobacterium lacus |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium lacus Turenne et al. 2002.
Species description is based on a single strain.
Mycobacterium, Mycobacterium lacus Turenne et al. 2002.
Species description is based on a single strain.
Acid-fast, large bacilli with prominent beading. Does not form aerial hyphae or spores.
Colonies on Lowenstein-Jensen medium are small, dry, and non-chromogenic.
Colonies on Middlebrook 7H10 agar are small, non-pigmented, and smooth to rough
with a slightly irregular edge. Younger colonies appear slightly transparent. Visible
growth appears in 2 weeks at 37-42 ºC from dilute inocula, but requires 3-4 weeks at
25-30 ºC. Temperature range for growth is 25-42 ºC (optimum 37 ºC). Does not grow
on media supplemented with 5% (w/v) NaCl or on MacConkey agar (without crystal
violet).
Colonies on Middlebrook 7H10 agar are small, non-pigmented, and smooth to rough
with a slightly irregular edge. Younger colonies appear slightly transparent. Visible
growth appears in 2 weeks at 37-42 ºC from dilute inocula, but requires 3-4 weeks at
25-30 ºC. Temperature range for growth is 25-42 ºC (optimum 37 ºC). Does not grow
on media supplemented with 5% (w/v) NaCl or on MacConkey agar (without crystal
violet).
Isolated from synovial tissue of a 68-year-old female patient with bursitis of the right elbow thought to be due to trauma whilst
swimming in a lake.
Susceptible (MIC) to amikacin (0.1 µg/ml), capreomycin (5.0 µg/ml), clarithromycin (2.0 µg/ml), ciprofloxacin (2.0 µg/ml), ethambutol
(2.0 µg/ml), ethionamide (2.5 µg/ml), isoniazid (0.1 µg/ml), kanamycin (2.5 µg/ml), levofloxacin (0.5 µg/ml), ofloxacin (1.0 µg/ml),
rifabutin (0.12 µg/ml), rifampin (0.5 µg/ml), Variable resistance to streptomycin (8 μg/ml).
swimming in a lake.
Susceptible (MIC) to amikacin (0.1 µg/ml), capreomycin (5.0 µg/ml), clarithromycin (2.0 µg/ml), ciprofloxacin (2.0 µg/ml), ethambutol
(2.0 µg/ml), ethionamide (2.5 µg/ml), isoniazid (0.1 µg/ml), kanamycin (2.5 µg/ml), levofloxacin (0.5 µg/ml), ofloxacin (1.0 µg/ml),
rifabutin (0.12 µg/ml), rifampin (0.5 µg/ml), Variable resistance to streptomycin (8 μg/ml).
Undetermined.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375. - Turenne C, Chedore P, Wolfe J, Jamieson F, Broukhanski G, May K, Kabani A. Mycobacterium lacus sp. nov., a novel slowly
growing, non-chromogenic clinical isolate. Int J Syst Evol Microbiol 2002; 52:2135-2140.
Positive results for acid phosphatase (weak reaction), arylsulfatase (weak reaction at 3 and 14 days), nitrate reduction, pyrazinamidase
(4 and 7 days), tellurite reduction, Tween 80 hydrolysis (weak reaction), and urease.
Negative results for acetamidase, nicotinamidase, succinamidase, heat-stable catalase (68 ºC), semiquantitative catalase test, iron
uptake, and niacin accumulation.
(4 and 7 days), tellurite reduction, Tween 80 hydrolysis (weak reaction), and urease.
Negative results for acetamidase, nicotinamidase, succinamidase, heat-stable catalase (68 ºC), semiquantitative catalase test, iron
uptake, and niacin accumulation.
(c) Costin Stoica
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