Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium kyogaense Nouioui et al. 2018.
Acid-alcohol-fast rods. Gram-positive.
Yellow-orange colonies are formed under light and dark conditions on a range of
media, including Lowenstein-Jensen, Middlebrook 7H10, PMG and TSA agar after
5 days at 37 ºC. Optimal growth occurs at 37 ºC and at pH 7.0. Can grow in 20 ºC, but
not in 4, 15, 42 or 45 ºC. No growth in 5% NaCl. Aerobic.
Isolated from mud from Kyoga Lake in Uganda.
- Nouioui I, Brunet LR, Simpson D, Klenk HP, Goodfellow M. Description of a novel species of fast growing mycobacterium:
Mycobacterium kyogaense sp. nov., a scotochromogenic strain received as Mycobacterium vaccae. Int J Syst Evol Microbiol 2018;
- BacDive in 2019: bacterial phenotypic data for High-throughput biodiversity analysis. Reimer, L. C., Vetcininova, A., Sardà
Carbasse, J., Söhngen, C., Gleim, D., Ebeling, C., Overmann, J. Nucleic Acids Research; database issue 2019.
Positive results for arylsulfatase (3 and 14 days), acid and alkaline phosphatase, catalase, trypsin, esterase (C4), cystine
arylamidase, esterase lipase (C8), alpha-galactosidase, alpha-glucosidase, beta-glucuronidase, lipase (C 14), leucine arylamidase,
naphthol-AS-BI-phosphohydrolase, tellurite reduction, Tween 80 hydrolysis, and urease.
Can utilize as sole carbon source butyric acid, alpha-ketobutyric acid, D-arabitol, D-fructose-6-phosphate, D-galactose, N-acetyl-D-
glucosamine and alpha-keto-glutaric acid.
Negative results for thermo-stable catalase (68 ºC), alpha-chymotrypsin, beta-galactosidase, beta-glucosidase, alpha-fucosidase, N-
acetyl-beta-glucosaminidase and alpha-mannosidase.
No utilization of citric acid, malic acid, gelatin, D-cellobiose, L-fucose, D-galactose, beta-gentiobiose, D-maltose, D-trehalose,
D-turanose, L-lactic acid, D-sorbitol, D-turanose, acetoacetic acid, D-glucuronic acid and quinic acid. (see Nouioui's paper for more).
(c) Costin Stoica