Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium kumamotonense Masaki et al. 2007.
Member of the Mycobacterium terrae complex.
Species description is based on a single isolate.
Acid-alcohol-fast, short rods. No spore or capsule production.
Colonies are 1-2 mm in diameter, smooth, nonchromogenic, and raised with round
or lobate regular margins on Middlebrook 7H9 agar and on 2% Ogawa medium after
incubation for 7-14 days. Neither spores nor capsules are observed. Temperature
range for growth is 25-42 ºC. Growth occurs in culture medium with hydroxylamine.
Does not grow on media supplemented with 5% (w/v) NaCl or 0.2% picrate.
Isolated from sputum. One isoalte from Stone Moroko intestine (Pseudorasbora parva). Susceptible to amikacin, clarithromycin,
ethambutol, ethionamid, levoﬂoxacin, rifampicin, and streptomycin. Resistant to isoniazid.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Masaki T, Ohkusu K, Hata H, Fujiwara N, Iihara H, Yamada-Noda M, Nhung PH, Hayashi M, Asano Y, Kawamura Y, et al.
Mycobacterium kumamotonense Sp. Nov. recovered from clinical specimen and the first isolation report of Mycobacterium
arupense in Japan: Novel slowly growing, nonchromogenic clinical isolates related to Mycobacterium terrae complex. Microbiol
Immunol 2006; 50:889-897.
- Fanourios Kontos, Dimitrios Nikitas Mavromanolakis, Marina Chari Zande, Zoe Georgios Gitti. Isolation of Mycobacterium
kumamotonense from a patient with pulmonary infection and latent tuberculosis. Indian Journal of Medical Microbiology 2016, 34,
- Mrlik V, Slany M, Kubecka J, Seda J, Necas A, Babak V, Slana I, Kriz P, Pavlik I. 2012. A low prevalence of mycobacteria in
freshwater fish from water reservoirs, ponds and farms. J. Fish Dis. 35:497–504. doi:10.1111/j.1365-2761.2012.01369.x.
Positive results for acid phosphatase, arylsulfatase (14 days), leucine, and cystine arylamidase, nitrate reduction, and Tween 80
Negative results for arylsulfatase (3 days), beta-galactosidase, alpha- and beta-glucosidase, and urease.
(c) Costin Stoica