Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium kubicae Floyd et al. 2000.
Member of the Mycobacterium simiae complex.
Strong acid-alcohol-fast rods, frequently bent with occasional coccoid forms (0.25-7.0
x 0.25-0.5 μm. No branching or aerial mycelia production.
Colonies on Lowenstein-Jensen and Middlebrook 7H11 agar, after 3-4 weeks
incubation, are yellow-colored and scotochromogenic. Smooth, domed growth occurs
on Middlebrook agar and film-like growth is seen on Lowenstein-Jensen medium.
Temperature range for growth is 28-37 ºC on Middlebrook agar, but for most isolates
is confined to 33-37 ºC on Lowenstein-Jensen medium. Does not grow at 42 ºC.
Isolated from sputum, from bronchial washings and other sources.
Susceptible to isoniazid (1 µg/ml), ethambutol (10 µg/ml). Resistant to streptomycin (8 µg/ml).
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Floyd MM, Gross WM, Bonato DA, Silcox VA, Smithwick RW, Metchock B, Crawford JT, Butler WR. Mycobacterium kubicae sp. nov.,
a slowly growing, scotochromogenic Mycobacterium. Int J Syst Evol Microbiol 2000; 50:1811-1816.
Positive results for semi-quantitative catalase, and Tween 80 hydrolysis (contradictory results in Floyd's original paper - admin note).
Negative results for arylsulfatase (3 days), nitrate reduction, and urease.
Variable results for arylsulfatase.
(c) Costin Stoica